摘要
为研究油菜素内酯(BR)对苹果矮化砧木T337株高调控机制的影响,以T337盆栽苗为试材,利用RT-PCR技术从T337中分离出BR合成关键基因MdCBB1.1和MdCBB1.2,二者的开放阅读框均为1 707bp,分别编码一个568个氨基酸的蛋白,分子质量分别为6.624 1×104 ku和6.622 8×104 ku。生物信息学分析结果显示,MdCBB1.1和MdCBB1.2基因编码的蛋白含有FAD_binding_4和GlcD 2个明显的结构域,为非核蛋白,属于植物CBB1蛋白家族。表达分析结果显示,MdCBB1.1和MdCBB1.2基因在T337不同组织均有表达,但是顶梢和根中的表达量明显高于其他组织(P<0.05)。并且外源芸苔素内酯(BL)处理能够上调二者在木质部中的表达(P<0.05)。
To investigate the mechanism of brassinolide （BR） affecting the height of apple dwarf root- stock T337, T337 seedlings were used for research. Full-length MdCBB1. I and MdCBB1. 2 genes were isolated from T337 by using RT-PCR technology. Both MdCBB1. I and MdCBB1.2 , playing a key role in BR biosynthesis, comprises a 1 707 bp open reading frame, encoding a 568 amino acids pro- tein with the molecular mass of 6. 624 1 X 10^4 ku and 6. 622 8 X 10^4 ku, respectively. Bioinformatics analysis revealed that the predicted protein is not nucleoprotein and including the typical sequences of CBB1 family, the FAD_binding_4 and GlcD domain. The expression analysis indicated that bothMd- CBB1. I andMdCBB1.2 genes expressed in different tissues of T337, hut the expression amount dif- fered significantly （P〈0.05）. The higher level of the expression was detected in shoot tip and root. Moreover, in xylem, the transcript level ofMdCBB1.1 andMdCBB1.2 were increased by the exoge- nous BL （P〈0.05）.
出处
《西北农业学报》
CAS
CSCD
北大核心
2016年第9期1365-1370,共6页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家科技支撑计划(2013BAD20B03)
国家星火计划(2014GA850002)
国家现代农业(苹果)产业技术体系建设专项(CARS-28)
国家苹果改良中心杨凌分中心
陕西省农业科技创新与攻关(2015NY114)
陕西省果业发展协同中心和西北农林科技大学试验示范站科技创新与成果转化项目(NYY2013-74)~~
