内皮素-1对牙周膜干细胞肿瘤坏死因子表达的影响被引量：1

Effect of Endothelin-1 on Expression of Tumor Necrosis Factor of Periodontal Ligament Stem Cells

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摘要目的:探讨内皮素-1(endothelin-1,ET-1)对牙周膜干细胞(periodontal ligament stem cells,PDLSCs)肿瘤坏死因子(Tumor Necrosis Factor,TNF-α)表达的影响。方法:体外培养鉴定牙周膜干细胞,加入不同浓度(1,10,100nmol/L)的ET-1培养12、24、72h,以未作任何处理的牙周膜干细胞为对照,采用ELISA及Western blot检测ET-1作用下牙周膜干细胞TNF-α分泌及蛋白表达的改变。结果:与对照组相比,ET-1对牙周膜干细胞TNF-α分泌及蛋白表达具有显著促进作用,且促进作用具有剂量依赖性及时间依赖性。结论:内皮素-1可以诱导牙周膜干细胞分泌TNF-α,而且这种促进作用呈浓度依赖性及时间依赖性。 Objective：To investigate how endothelin-1affects the expression of tumor necrosis factor（TNF-α）in periodontal ligament stem cells（PDLSCs）.Methods：The PDLSCs were stimulated with endothelin-1（1,10,100nmol/L）for 12,24,and 72 hours.The untreated PDLSCs served as normal control.The effect of ET-1stimulation on TNF-αprotein levels was evaluated by ELISA and Western blot.Results：The TNF-αprotein levels were enhanced by ET-1stimulation in a dose-dependent and time-course manner.Conclusion：These results indicate that ET-1is involved in the regulation of TNF-αexpression in PDLSCs and suggest that ET-1signaling may be a therapeutic target for treating TNF-α-dependent inflammatory responses.

作者梁莉余继锋周威刘洪臣

机构地区解放军首都医科大学附属北京儿童医院眼科解放军总医院口腔医学研究所

出处《口腔医学研究》 CAS CSCD 北大核心 2016年第11期1165-1167,共3页 Journal of Oral Science Research

基金国家自然科学基金项目(编号:81500821)

关键词牙周炎牙周膜干细胞内皮素-1 肿瘤坏死因子 Periodontitis Periodontal ligament stem cells Endothelin-1 Tumor necrosis factor

分类号 R781.42 [医药卫生—口腔医学]

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参考文献2

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