摘要
目的:探讨水溶性辅酶Q10(CoQ10)对鱼藤酮诱导的帕金森病(PD)大鼠神经变性的影响。方法:24只成年雌性SD大鼠随机分成3组(n=8):对照组皮下注射葵花油乳剂液,PD组皮下注射鱼藤酮,PD治疗组在诱导PD后接受水溶性CoQ10治疗。8周后,酶联免疫吸附测定法(ELISA)测定血清转化生长因子-β1(TGF-β1)、单核细胞趋化蛋白-1(MCP-1)、脑源性神经营养因子(BDNF)水平;Western-blot检测survivin、caspase-3、caspase-8、caspase-9的表达水平;免疫组化观察大脑激活型caspase-3的表达。结果 :水溶性CoQ10能明显降低caspase-3、caspase-9的表达水平,升高survivin的表达水平,但caspase-8的表达水平无影响,能减少激活性caspase-3的阳性细胞水平。结论:水溶性CoQ10能降低鱼藤酮引起的炎症反应,抑制凋亡蛋白的表达,促进多巴胺能神经元分泌抗凋亡蛋白,增加神经营养因子的含量,以阻止或延缓进行性神经变性的发生,但不可逆转PD引起的神经变性。
Objective To discuss the influence of orally delivered water soluble coenzyme QlO （CoQlO） on on-going neurodegeneration in rats exposed to rotenone. Methods Twenty-four adult female SD rats were randomly divided into 3 groups （n = 8）. Control group was injected subcutaneously sunflower oil, PD group subcutaneous injection rotenone to PD, and PD treatment group received water-soluble CoQ10 treatment after induction of PD. After 8 weeks, serum levels of transforming growth factor-β1 （TGF-β1）, monoeyte chemoattractant protein-1 （MCP-1） and brain-derived neurotrophic factor （BDNF） were detected by enzyme-linked immunosorbent assay （ELISA） kit; Western blot analysis was conducted for the expression level of survivin, caspase-3, caspase-8 and caspase-9; the expression of activated easpase-3 was observed in brain by immunohistoehemieal staining. Results Water soluble CoQ10 could significantly decrease the level of caspase-3 and caspase-9, with increasing the level of survivin, but the expression level of caspase-8 did not change, and the expression of caspase-3 positive cells was reduced. Conclusions Water-soluble CoQ10 could reduce rotenone-induced inflammation, inhibit the expression of apoptotic protein, promote dopaminergie neurons secrete anti-apoptotic protein and increase the amount of neurotrophic factors to prevent or delay the occurrence of on-going neurodegeneration, hut it has no irreversible effect on the neurodegeneration of PD.
出处
《实用医学杂志》
CAS
北大核心
2016年第22期3637-3641,共5页
The Journal of Practical Medicine
基金
国家自然科学基金项目(编号:81460179)
