摘要
[ Objective ] The paper was to explore effects of Tomato aspermy virus (TAV) -free on chlorophyll content and nitrogen metabolism of Chrysanthemum morifolium Ramat. 'Huaibai', in order to provide a theoretical basis for field popularization and application of TAV-free ' Huai bai' plantlets. [ Method] With previously acquired TAV-free test-tube plantlets of ' Huaibai' as the experimental materials and its ordinary test-tube plantlets as the control (CK), chlorophyll content and some indexes related to nitrogen metabolism in leaves were determined during subculture. [ Result] During subculture, the contents of chlorophyll a and chlorophyll b in leaves of CK and TAV-free ' Huaibai' test-tube plantlets increased with the subculture days. Both realized the largest increase at the 22'h day of subculture, and their increase amplitudes were 5.24% and 41.69%, respectively. Compared with CK, the chlorophyll a and chlorophyll b contents of TAV-free test-tube plantlets increased at the same time point of subculture, with significant difference (P 〈0.05) or extremely significant difference (P 〈0.01 ). Further- more, the nitrate reductase (NR) activity, free proline and soluble protein contents of TAV-free ' Huaibai' were all significantly higher than those of CK, and the highest increase amplitudes were 27.99% ( subculture for 22 d), 28.10% ( subculture for 22 d) and 5.89% ( subculture for 38 d), respectively. [ Conclusion] TAV-free could effectively improve chlorophyll content and nitrogen metabolism level of ' Huaibai' , and was conducive to its growth and development.
[ Objective ] The paper was to explore effects of Tomato aspermy virus (TAV) -free on chlorophyll content and nitrogen metabolism of Chrysanthemum morifolium Ramat. 'Huaibai', in order to provide a theoretical basis for field popularization and application of TAV-free ' Huai bai' plantlets. [ Method] With previously acquired TAV-free test-tube plantlets of ' Huaibai' as the experimental materials and its ordinary test-tube plantlets as the control (CK), chlorophyll content and some indexes related to nitrogen metabolism in leaves were determined during subculture. [ Result] During subculture, the contents of chlorophyll a and chlorophyll b in leaves of CK and TAV-free ' Huaibai' test-tube plantlets increased with the subculture days. Both realized the largest increase at the 22'h day of subculture, and their increase amplitudes were 5.24% and 41.69%, respectively. Compared with CK, the chlorophyll a and chlorophyll b contents of TAV-free test-tube plantlets increased at the same time point of subculture, with significant difference (P 〈0.05) or extremely significant difference (P 〈0.01 ). Further- more, the nitrate reductase (NR) activity, free proline and soluble protein contents of TAV-free ' Huaibai' were all significantly higher than those of CK, and the highest increase amplitudes were 27.99% ( subculture for 22 d), 28.10% ( subculture for 22 d) and 5.89% ( subculture for 38 d), respectively. [ Conclusion] TAV-free could effectively improve chlorophyll content and nitrogen metabolism level of ' Huaibai' , and was conducive to its growth and development.
基金
Supported by National Natural Science Foundation of China(31372105)
Subproject of Special Research of National Traditional Chinese Medicine Industry(201407005-08)
Support Project of Science and Technology Innovation Teams in Colleges and Universities of Henan Province(15IRTSTHN020)
Project for Construction of Innovative Science and Technology Talent Team in Henan Province(C20130037)