期刊文献+

BOPIM-dma作为BSA Site Ⅰ特异性探针的研究及其应用

BOPIM-dma as BSA Site Ⅰ Specific Probe for Drug Binding Study
下载PDF
导出
摘要 本文选取具有扭转的分子内电荷转移(TICT)特性BOPIM-dma作为BSAsiteⅠ特异性探针,研究了9种药物与BSA结合性质.溶剂化荧光猝灭的BOPIM—dma在结合到BSA的结合点位SiteⅠ后在500nm处发射出强绿色荧光.选取两类作用于BSA SiteⅠ的药物,包括黄酮类药物:槲皮素(Quercetin)、山奈酚(Kaempeferol)、黄芩素(Baicalein)、柚皮素(Naringenin)、芹菜素(Apigenin)、木犀草素(Iuteolin);抗炎药:舒林酸(Sulindac)、吲哚美辛(Indomethacin)、托美丁钠(Tolmetin Sodium Saltdihydrate).实验结果与文献对照,仅槲皮素、山奈酚因为本身-500nm处有荧光而出现偏差,其他活性顺序与文献是一致的.并且BOPIM—dma/BSA体系发绿光,增强了实验的可视性.这也表明:BOPIM—dma作为BSA siteⅠ特异性探针研究作用于该位点的药物与BSA之间的相互作用性质是可靠的. BOPIM-dma with twisted intramolecular charge transfer (TICT) character was used to act as BSA Site I fluorescence specific probe for studying of the interaction between BSA and drugs. BOPIM-dma emits strong green fluorescence in BSA (- 500 nm). The binding of drug to BSA quenched the fluorescence of BOPIM-dma because of dissociation of BOPIM-dma from hydrophobic pocket of BSA Site I . Two kinds of drugs, including {lavonoids:quercetin, kaempeferol, baicalein, naringenin, apigenin, iuteolin, and anti-in- flammatory.sulindac, indomethacin and tolmetin sodium salt dehydrate were selected for the binding investi-gation. It is shown that the binding constant of studied drugs to BSA obtained from our results are consistent with the data have been reported. Thus, BOPIM-dma is suitable for the specific probe for BSA Site I to study the interaction between drugs and BSA, except of drugs which emit fluorescence at - 500 nm themselves.
出处 《三峡大学学报(自然科学版)》 CAS 2016年第6期108-112,共5页 Journal of China Three Gorges University:Natural Sciences
基金 国家自然科学基金(21473101)资助
关键词 荧光探针 BOPIM-dma 牛血清蛋白(BSA) 结合位点Ⅰ 药物 结合常数 fluorescent probe BOPIM-dma bovine serum albumin (BSA) site Ⅰ drugs bindingconstant
  • 相关文献

参考文献5

二级参考文献73

共引文献135

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部