摘要
目的:通过对海藻酸钠链段羟基位点改性制备甲氧基聚乙二醇(MPEG)原位共价修饰的海藻酸钠/壳聚糖(AC)微胶囊,在保证MPEG修饰微胶囊机械强度不受影响的基础上,有效提高表面MPEG修饰密度,实现兼具良好机械稳定性及抗蛋白性能的微胶囊制备方法。方法:利用溴化氰对海藻酸钠羟基进行活化并将末端氨基的点击化学linker(BAT)接枝在主链上进而制备MPEG原位共价修饰微囊A_(B(OH))CP_N,用球磨法表征微囊机械强度,用Ig G和Fgn为模型考察微囊表面抗蛋白吸附性能,以L929细胞在其二维模拟平板膜上的黏附情况作为衡量指标,考察MPEG修饰微胶囊表面细胞粘附情况,并最终通过体内移植考察MPEG修饰微囊的生物相容性。结果:基于海藻酸钠羟基位点的MPEG原位共价修饰微胶囊能够实现与常规条件制备的微胶囊接近的机械强度;同时与对照组相比Ig G吸附量降低87.4%,Fgn吸附量降低75.5%,实现了良好的抗蛋白吸附性能;二维模拟平板膜表面L929细胞粘附情况显著改善,细胞粘附数与对照组相比降低了76.9%;体内移植结果证明MPEG修饰微囊细胞粘附极少,微囊与纤维层分离明显。结论:基于海藻酸钠羟基位点的MPEG原位修饰能够实现兼具良好机械稳定性及抗蛋白吸附性能的微胶囊。
Objective： To improve the methoxyl polyethylene glycol（MPEG） surface graft density significantly on the basis of maintaining good mechanical strength, in-situ MPEG covalently modified alginate/chitosan（AC） microcapsules were prepared based on the modified hydroxyl groups of alginate. Methods： A click chemistry linker（BAT） was grafted on the activated hydroxyl groups of alginate which was obtained by using the cyanogen bromide activation and then the in-situ MPEG covalently modified alginate/chitosan microcapsules（A（B（OH））C（PN）） were prepared. The mechanical stability of microcapsules was measured by rupture ratio after ball milling, and the protein resistance of MPEG modified microcapsules was evaluated by immunoglobulin G（Ig G） and fibrinogen（Fgn）. Then, the adhesion of cells on the modified AB（OH）CPNmembrane was investigated using L929 as model cells. Results： MPEG modified microcapsules were prepared with their mechanical stability similar to the control groups obtained under conventional preparation condition. Compared with control group, the adsorption of Ig G and Fgn on the surface of MPEG modified microcapsules was reduced by 87.4% and 75.5%, respectively, which showed great protein resistance ability. The adhesion of L929 on the A（B（OH））C（PN）flat membrane was improved significantly which was proved by the result that calculated number of adhered L929 was reduced by 76.9% compared to AC membrane. The in-vivo transplantation results proved the slighter immune response caused by AB（OH）CPNmicrocapsules. Conclusions： The mechanical stability and biocompatibility of MPEG modified microcapsules obtained based on the activation of hydroxyl groups of alginate were greatly improved.
出处
《现代生物医学进展》
CAS
2016年第33期6401-6405,共5页
Progress in Modern Biomedicine
基金
中国海洋公益性行业科研专项(201405015-3)
关键词
微胶囊
MPEG
机械稳定性
抗蛋白吸附
抗细胞粘附
Microcapsules
MPEG
Mechanical stability
Protein adsorption resistance
Cell adhesion resistance
