摘要
为研究金黄色葡萄球菌疫苗候选抗原SirH和StbA及其抗血清的免疫保护作用,分别用纯化蛋白StbA、SirH以及甲醛灭活的金黄色葡萄球菌为免疫原免疫小鼠,收集抗血清,测定血清效价,然后用致死剂量的金黄色葡萄球菌攻击,评价两种抗原的免疫保护作用。分别用SirH和StbA抗血清免疫小鼠,4 h后用致死剂量的金黄色葡萄球菌攻击,评价抗血清的保护作用。结果显示,StbA和SirH抗血清效价均为1：1 638 400。金黄色葡萄球菌攻击主动免疫小鼠,SirH纯化蛋白免疫组（60%）和StbA纯化蛋白免疫组（70%）存活率均明显高于对照组（0%）（P〈0.05）,金黄色葡萄球菌全菌免疫组（20%）存活率与对照组（0%）差异不具备显著统计学意义（P〉0.05）。SirH纯化蛋白免疫组（60%）和StbA纯化蛋白免疫组（70%）存活率均高于金黄色葡萄球菌全菌免疫组（20%）。将抗血清免疫小鼠后,用金黄色葡萄球菌腹腔内注射攻击小鼠,免疫组小鼠16-18 h内全部死亡,而对照组小鼠在攻毒后12-14 h内全部死亡。结果表明,SirH和StbA是具有潜力的保护性抗原,但其抗血清都不具有保护作用。
In order to investigate immunoprotection of vaccine antigen candidates SirH and StbA and their specific antisera against Staphylococcus aureus infection,mice were respectively immunized with the purified recombinant proteins and antisera of candidate antigens,and then the immunized mice were challenged with the lethal dose of Staphylococcus aureus.The result showed that antibody titers of mouse sera immunized with the recombinant SirH or StbA were both 1：1 638 400.All actively immunized mice were injected with a lethal dose of Staphylococcus aureus.The survival rates in the SirH-immunized group（60%） and the StbA-immunized group（70%）were both significantly higher than that in the control group（0%）（P〈0.05）.The survival rates between the whole-cell bacterial vaccine group（20%） and the control group（0%） have no significant difference（P〈0.05）.The survival rates in the SirH-immunized group（60%）and the StbA-immunized group（70%） were both higher than in the whole-cell bacterial vaccine group（20%）.Mice in the antiserumimmunized group and control groups were challenged with a lethal dose of Staphylococcus aureus.The antiserum-immunized mice were all dead within 16 to 18 hours,whereas mice in control group were dead within 12 to 14 hours after challenge.In conclusion,SirH and StbA are both potential protective antigens,but the SirH- or StbA-specific antiserum could not protect mice from lethal challenge with Staphylococcus aureus.
出处
《中国动物传染病学报》
CAS
北大核心
2016年第4期48-51,共4页
Chinese Journal of Animal Infectious Diseases
基金
国家自然科学基金项目(31360084)
贵州省科技合作计划(黔科合LH字[2015]7318)
关键词
金黄色葡萄球菌
疫苗抗原
抗血清
免疫保护
Staphylococcus aureus
vaccine antigens
antisera
immunoprotection
