摘要
[目的]谷子属于耐旱杂粮作物,但谷子响应干旱胁迫的相关基因分子机制仍不清楚。为了明确谷子干旱胁迫是否与淀粉代谢关键酶基因相关,探索谷子抗旱的分子机制。[方法]运用生物信息学初步分析了谷子该家族基因的基本序列特征、启动子元件预测。构建该基因家族直系同源系统进化树,分析与其他物种同源蛋白的亲缘关系。基于数字基因表达谱数据分析了PEG模拟干旱条件下,该基因家族在谷子抗旱品种‘勾勾母鸡咀’和敏感性品种‘晋汾16’的表达特征。[结果]谷子全基因组调查表明该基因家族有五个成员,暂且命名为SiSBD1、SiSBD2、SiSBD3、SiSBD4和SiSBD5。序列分析表明,该基因家族成员编码的蛋白序列长度范围为360-949个氨基酸残基;蛋白分子量范围为39-108kD,蛋白质等电点为4.18-6.72。启动子元件分析鉴定出与抗旱相关的顺式作用元件,主要包含：与MYB抗旱基因调控相关,与MeJA、ETH、SA、ABA等在抗旱中起重要作用的植物激素相关,与植物防御和非生物胁迫响应相关。系统进化树表明,谷子SiSBD1基因与高粱和玉米同源蛋白SBD聚为一类,谷子SiSBD2基因与玉米SBD2、SBD3基因的聚为一类,其他3个基因归为一类。进一步分析PEG胁迫下谷子淀粉结合域蛋白基因家族表达,结果表明：SiSBD1在抗旱品种‘勾勾母鸡咀’中表达增加,在敏感性品种‘晋汾16’中表达降低,而其他4个基因的表达在这两个品种中均降低。[结论]该研究结果暗示了淀粉代谢关键酶SDB基因家族与干旱胁迫相关,可为谷子抗旱育种提供一定的理论基础。
Foxtail millet is drought-tolerant crop,but the molecular mechanisms related to drought-tolerant are still unclear.[Objective]This study aimed to determine whether the drought-tolerant character of foxtail millet was related to the key enzymes of starch metabolism and lay the foundation for the further study on the molecular mechanism of drought resistance in foxtail millet and drought resistance breeding of foxtail millet.[Methods]Bioinformatics analysis was applied to analyze the basic sequence characteristics of SBDgene family and the promoter elements in foxtail millet,built the phylogenetic tree of the gene family and determine the evolutionary relationships with other species.Based on digital gene expression profiling data,we analyzed the expression characteristics of SiSBD gene in drought-tolerant variety ‘Gou Gou Mu Ji Ju（GG）＇and drought-sensitive variety‘Jin Fen 16（JF-16）＇under PEG stress.[Results]The genome sequence data of foxtail millet showed that there were five members of SiSBD gene family named SiSBD1,SiSBD2,SiSBD3,SiSBD4 and SiSBD5.The sequence analysis indicated that the length of encoded protein sequence was in the range of 360~949amino acid residues,the molecular weight was 39 ~ 108 KD,the isoelectric point was 4.18~6.72.The Cis-acting elements associated with drought tolerance were identified including Cis-acting elements related to MYB gene regulation,plant hormones as MEJA,ETH,SA,ABA,and plant defense and abiotic stress response regulation.The phylogenetic tree showed that SiSBD1 and SBDin Sorghum bicolor and Zea mays were classified to the same group;SiSBD2and SBD2,SBD3 in Zea mays were classified to the same group;SiSBD3,SiSBD4 and SiSBD5 were classified to the same group.The analysis results of expression of starch binding domain gene family in foxtail millet under PEG stress showed that the expression of SiSBD1 increased in drought-tolerant variety‘Gou Gou Mu Ji Ju＇and decreased in drought-sensitive variety ‘Jin Fen 16＇,the expression of other four genes decreased in both varieties.[Conclusion]These results suggested that the SDBgene families related with response to drought stress and laid a theoretical foundation for breeding drought tolerant variety in the future.
出处
《山西农业大学学报（自然科学版）》
CAS
2016年第12期855-859,878,共6页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
国家自然科学基金(31471556)
关键词
谷子
STBD
CGTASE
GAA
抗旱性
分子机制
Setaria italica
SBD gene family
CGTase
GAA
Drought resistance
Molecular mechanism
