期刊文献+

超高效液相色谱-串联质谱法测定饲料中的恩诺沙星和喹乙醇药物的含量 被引量:7

Determination of enrofloxacin and olaquindox in feeds by ultra performance liquid chromatography-tandem mass spectrometry
下载PDF
导出
摘要 试验建立了同时测定饲料中恩诺沙星和喹乙醇药物含量的超高效液相色谱-串联质谱(UPLC/MS/MS)分析方法。采用80%乙腈(含1%甲酸)作为样品的提取溶液,经HLB固相萃取柱净化后上机测定。在正离子模式下通过两对离子对进行定性和定量分析。结果显示,恩诺沙星和喹乙醇在5-100μg/l范围内线性关系良好,相关系数r均大于0.998;空白饲料在0.1、0.2、0.5mg/kg的添加浓度上的回收率均在70%-90%之间;批内、批间相对偏差(RSD)均小于20%;本方法对饲料中恩诺沙星和喹乙醇的定量限为0.1mg/kg(S/N〉10),检测限为0.05mg/kg(S/N〉3)。 A method based on ultra performance liquid chromatography-tandem mass spectrometry has been developed for the determination of enrofloxacin and olaquindox in feeds. The analytes were ex- tracted with 80% acetonitrile containing 1% formic acid, and were cleaned by the HLB cartridge. The purified extracts were analysed by UPLC-MS/MS under the mode of positive electrospray ionization. The results indicated that in the range of 5 to 100μg/l, the correlations of enrofloxacin and olaquin- dox were greater than 0.998. The average recoveries of enrofloxacin and olaquindox at three concentra- tion levels of 0.1, 0.2 mg/kg and 0.5 mg/kg ranged from 70% to 90%. The RSD were less than 20%. The limits of quantity(S/N〉10), and limits of detection(S/N〉3) by the present method for the enrofloxacin and olaquindox were found to be 0.1 mg/kg and 0.05 mg/kg respectively.
机构地区 驻马店市畜牧局
出处 《饲料工业》 北大核心 2016年第22期53-57,共5页 Feed Industry
关键词 恩诺沙星 喹乙醇 饲料 液相色谱-串联质谱 enrofloxacin olaquindox feeds ultra performance liquid chromatography- tandem massspectrometry
  • 相关文献

参考文献8

二级参考文献163

共引文献126

同被引文献67

引证文献7

二级引证文献33

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部