摘要
采用超临界二氧化碳提取葫芦巴提取物,利用气相色谱-质谱法分析提取物的化学成分,并通过面积归一化法分析各成分的相对含量。从葫芦巴超临界二氧化碳提取物中共鉴定出49个化合物,占提取物总量的88.37%,其中亚油酸(45.29%)含量最高。利用分光光度法测定葫芦巴超临界二氧化碳提取物对DPPH自由基的清除作用,其清除作用良好,半清除浓度为4.57 mg·m L-1,且清除率与样品浓度呈量效关系。葫芦巴超临界提取物营养成分丰富、安全无毒、有特殊香气,在食品、香料等领域可广泛应用。
Supercritical fluid extraction（SFE）using carbon dioxide was employed to extract Trigonella foenum- graecum L.and the chemical components were determined by GC-MS. The relative contents of the chemical components were determined with peak area normalization method. Forty-nine compounds, which occupied 88.37% of total constituents were identified. The major constituent was linoleic acid （45.29%）. The scavenging capacity against DPPH free radical of SFE extracts from Trigonella foenum-graecum L.was determined by spectre photometry.The extracts exhibited significant scavenging capacity against DPPH free radical in a concentration- dependent fashion with the SC5o value of 4.57 mg·mL-1.The SFE extracts was rich in nutrients, safe and non-toxic, and had special aroma.So it could be widely used in the fields of food and perfume.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第24期153-156,162,共5页
Science and Technology of Food Industry
基金
天然药物研究重庆市高校市级重点实验室开放基金项目(CQCM-2015-07)
重庆工商大学科研启动项目(2015-56-07)
重庆工商大学科研基金项目(2015)
关键词
葫芦巴
超临界CO2提取
气相色谱-质谱联用
DPPH自由基
Trigonella foenum-graecum L.
supercritical fluid extraction using carbon dioxide
GC- MS
DPPH freeradical
