摘要
以短梗五加茎为原料,采用超声波辅助提取短梗五加中总皂苷。研究料液比、乙醇体积分数、超声时间、超声功率四个因素对短梗五加中总皂苷提取效果的影响,在单因素实验的基础上,采用Box-Behnken Design实验,以总皂苷得率为响应值,建立回归模型并由此得出四个因素最优提取工艺组合为:料液比1∶50,乙醇体积分数68%,超声时间41 min,超声功率350 W,此条件下短梗五加总皂苷的得率为1.577%。实验真实值与模型预测值1.586%基本一致,表明响应面中心组合法合理可行,可以优化提取短梗五加总皂苷的工艺。
The root stem of Acanthopanax sessiliflorus（ Rupr. et Maxim.）Seem was used as the raw material, ultrasonic assisted extraction of total saponins from Acanthopanax sessiliflorus.The effect of material-to-liquid ratio, ethanol concentration, ultrasonic time, ultrasonic power on the extraction result of the total saponin of Acanthopanax sessiliflorus was studied. On the basis of single factor experiment, Box- Behnken Design was applied to build a regression model, with the yield rate of total saponin as response value. Establish regression model and according to this,the optimal extraction combination was follows：material-to-liquid ratio ]：50, ethanol concentration 68% ,ultrasonic time 4! min, ultrasonic power 350 W. In this condition,the yield of the total saponin was 1.577%.The true value of the experiment accords with the predictive value of the model was 1.586% ,show that response surface center combination method is reasonable and feasible, which can optimize the extraction process of total saponins from Acanthopanax sessiliflorus.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第24期272-277,共6页
Science and Technology of Food Industry
基金
国家自然科学基金面上项目(81274114)
关键词
短梗五加
总皂苷
超声提取
响应面法
Acanthopanax sessiliflorus
total saponin
ultrasonic extraction
response surface methodology
