摘要
目的研究牡荆苷对脑缺血再灌注损伤小鼠抗氧化酶的作用及机制。方法雄性昆明种小鼠70只,按随机数字表法分为正常对照组、假手术组、模型组、牡荆苷组(10、20、40 mg/kg)、尼莫地平组(0.6 mg/kg),采用线栓法制备脑缺血再灌注模型,牡荆苷组在缺血再灌注前连续腹腔给予牡荆苷3 d,缺血再灌注后,每天给药1次,连续给药7 d,检测小鼠神经功能损伤评分,采用试剂盒法检测脑组织、血清中丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、髓过氧化物酶(MPO)的活性。结果与假手术组比较,模型组神经功能损伤评分显著升高(P<0.01);与模型组比较,牡荆苷组神经功能损伤评分显著降低(P<0.01)。与假手术组比较,模型组脑组织、血清中MDA含量、MPO活性显著升高,SOD、GSH-Px、CAT活性显著降低,差异均有高度统计学意义(P<0.01);与模型组比较,尼莫地平组和牡荆苷组脑组织、血清中MDA含量、MPO活性显著降低,SOD、GSH-Px、CAT活性显著增强,差异有统计学意义(P<0.01或P<0.05)。与尼莫地平组比较,10、20 mg/kg牡荆苷组MDA含量和MPO活性显著上升,SOD、GSH-Px、CAT活性显著降低(P<0.05);40 mg/kg牡荆苷组与尼莫地平组比较差异无统计学意义(P>0.05)。结论牡荆苷对小鼠脑缺血再灌注损伤的保护作用可能通过抗氧化作用来实现。
Objective To study the effects and mechanism of vitexin on antioxidant enzymes in cerebral ischemia- reperfusion injury mice. Methods Seventy Kunming male mice were randomly divided into the normal control group, sham group, model group, vitexin group （10, 20, 40 mg/kg） and Nimodipine group （0.6 mg/kg）. Mice were prepared with cereral ischemia-reperfusion injury by suture method. Vitexin was intraperitoneally injected three days before cerebral isehemia, after ischemia-reperfusion, mice were given drugs once daily for seven consecutive days, then neurological deficits were determined. The contents of malondialdehyde （MDA）, the activities of superoxide dismutase （SOD）, cata- lase （CAT）, glutathione peroxidase （GSH-Px）, and nlyeloperoxidase （MPO） in brain tissue and serum were determined by the kits methods. Results Compared with sham group, the neurological score was remarkably increased in model group （P 〈 0.01）; compared with model group, the nemological scores in vitexin group were significantly decreased （P 〈 0.01）. Compared with sham group, the content of MDA and the activity of MPO in model group were increased, the activities of SOD, CAT, GSH-Px in brain tissue and serum were reduced, the differences were all highly statistically significant （P 〈 0.01）. Compared with model group, the content of MDA and the activity of MPO of brain tissue and serum in Nimodipine group and vitexin group were decreased, the activities of SOD, CAT, GSH-Px were strengthened, the diflerences were statistically significant （P 〈 0.01 or P 〈 0.05）. Compared with Nimodipine group, the content of MDA and the activity of MPO in 10, 20 mg/kg of vitexin group were increased, the activities of SOD, CAT, GSH-Px were reduced （P 〈 0.05）, there were no significant differences between 40 mg/kg of vitexin group and Nimodipine group （P 〉0.05）. Conclusion Vitexin against cerebral ischemia- reperfusion injury might through its anti-oxidative effect.
出处
《中国医药导报》
CAS
2016年第33期12-15,20,共5页
China Medical Herald
基金
河北省医学科学研究重点课题计划项目(20150474)
关键词
牡荆苷
脑缺血再灌注
抗氧化酶
Vitexin
Cerebral ischemia-reperfusion
Antioxidant enzymes
