摘要
目的:研究骨肉瘤组织中Gab2、IRX2表达量与细胞生长、迁移行为的相关性。方法:选择在我院手术切除的29例骨肉瘤组织和22例骨样骨瘤组织作为检测标本,抽提RNA并测定Gab2、IRX2及细胞生长、迁移相关基因的mRNA表达量,抽提蛋白并测定Gab2、IRX2及细胞生长、迁移相关基因的蛋白表达量。结果:骨肉瘤组织中Gab2、IRX2的mRNA含量和蛋白含量均显著高于骨样骨瘤组织;骨肉瘤组织中TAp73、Beclin1、Caspase-1的mRNA含量和蛋白含量均显著低于骨样骨瘤组织且与Gab2、IRX2的mRNA含量和蛋白含量呈负相关,STMN1、Survivin的mRNA含量和蛋白含量均显著高于骨样骨瘤组织且与Gab2、IRX2、CEACAM6、CD44v6、MMP-9的mRNA含量和蛋白含量呈正相关。结论:骨肉瘤组织中高表达的Gab2和IRX2能够抑制原癌基因的表达、增加促增殖和迁移基因的表达,进而促进骨肉瘤组织中细胞的生长、迁移。
Objective： To study the correlation of Gab2 and IRX2 expression with cell growth and migration behavior in osteosarcoma tissues. Methods： A total of 29 cases of osteosarcoma tissues and 22 cases of osteoid osteoma tissues surgically removed in our hospital were selected as the test specimens, RNA was extracted to determine the mRNA expression of Gab2 and IRX2 as well as cell growth- and migration-related genes, and protein was extracted to determine the protein expression of Gab2 and IRX2 as well as cell growth- and migration-related genes. Results： Gab2 and IRX2 mRNA content and protein content in osteosarcoma tissues were significantly higher than those in osteoid osteoma tissues; TAp73, Beclinl and Caspase-1 mRNA content and protein content in osteosarcoma tissues were significantly lower than those in osleoid osteoma tissues and negatively correlated with Gab2 and IRX2 mRNA content and protein content while STMN1 and Survivin mRNA content and protein con- tent were significantly higher than those in osteoid osteoma tissues and positively correlated with Gab2, IRX2, CEACAM6, CD44v6 and MMP-9 mRNA content and protein content. Conclusion： Highly expressed Gab2 and IRX2 in osteosarcoma tissue can inhibit the expression of proto-oncogenes and increase the expression of proliferation and migration genes to promote thecell growth and migration in osteosarcoma tissue.
出处
《海南医学院学报》
CAS
2016年第24期2944-2946,2950,共4页
Journal of Hainan Medical University
基金
湖北省卫生和计划生育委员会科研项目(WJ2015MB114)~~
