摘要
目的:考察吴茱萸对左金丸中盐酸小檗碱、盐酸巴马汀和盐酸药根碱在大鼠组织中分布动力学的影响,探讨吴茱萸的引经作用机制。方法:大鼠分别灌胃给予黄连、黄连吴茱萸合煎液,于给药后不同时间点采集组织样本,利用UPLCMS/MS测定组织中3种黄连生物碱类成分的经时变化,采用DAS 2.0软件计算组织分布动力学参数、相对摄取率(Re)和峰浓度比(Ce)。结果:左金丸组大鼠肝组织中盐酸小檗碱和盐酸巴马汀的药-时曲线下面积分别为(7 051.268±518.354),(2 179.139±195.617)ng·g-1·h,药峰浓度分别为(1 645.961±129.862),(383.889±19.526)ng·g-1,与黄连组比较显著增加。3种生物碱类成分的在肝中Re分别为1.338,1.475和1.065,Ce分别为1.548,1.751和1.369;肺中Re分别为0.985,0.780和0.930,Ce分别为0.834,1.051和0.905。结论:吴茱萸可能是通过增加黄连生物碱类成分在大鼠肝中分布和减少其在肺中分布来发挥引经作用。
Objective： To observe the effect of Euodiae Fructus on tissue distribution kinetics of berberine hydrochloride,palmatine hydrochloride and jatrorrhizine hydrochloride from Coptidis Rhizoma in rats.Method： Rats were administered respectively by Coptidis Rhizoma and codecoction of Coptidis Rhizoma and Euodiae Fructus. Tissue samples were collected at diferent time after administration. The variation of berberine hydrochloride,palmatine hydrochloride and jatrorrhizine hydrochloride with time was determined by UPLC-MS/MS. The kinetic parameters,relative uptake rate（ Re） and peak concentration ratio（ Ce） were calculated by DAS 2. 0 software. Result： In group of codecoction of Coptidis Rhizoma and Euodiae Fructus, AUC0-∞of berberine hydrochloride and palmatine hydrochloride distributed in the liver tissues were（ 7 051. 268 ± 518. 354）,（ 2 179. 139 ± 195. 617） ng·g-1·h,Cmaxwere（ 1 645. 961 ± 129. 862）,（ 383. 889 ± 19. 526） ng·g-1; compared with the Coptidis Rhizoma group, they were increased significantly. Re of these three alkaloids in liver were1. 338,1. 475 and 1. 065,Ce of them in liver were 1. 548,1. 751 and 1. 369,Re of them in lung were 0. 985,0. 780 and 0. 930, Ce of them in lung were 0. 834, 1. 051 and 0. 905. Conclusion： Euodiae Fructus has properties of reducing the distribution of alkaloids from Coptidis Rhizoma in the lung and increasing their distribution in the liver.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第23期89-93,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81403124)
关键词
吴茱萸
黄连
引经
组织分布
盐酸小檗碱
盐酸巴马汀
盐酸药根碱
Euodiae Fructus
Coptidis Rhizoma
leading action
tissue distribution
berberine hydrochloride
palmatine hydrochloride
jatrorrhizine hydrochloride
