摘要
目的探讨缺血前运动训练对大鼠脑局灶缺血后神经生长因子（NGF）及其受体TrkA表达水平的影响，以及该方法对脑局灶缺血后大鼠空间学习记忆功能的作用。方法雄性SD大鼠36只，随机数字表法分为3组：假手术组（Sham—MCAO，n=12）、脑局灶性缺血再灌注组（MCAO，n=12）、缺血前运动训练＋脑局灶性缺血再灌注组（EX＋MCAO，n=12）。EX＋MCAO组大鼠放置于滚笼装置进行规律运动训练4周后，采用大脑中动脉栓塞（middlecerebralarteryocclusion，MCAO）法制备短暂局灶性脑缺血再灌注模型．参照Garcia等的方法对大鼠进行神经功能缺损评分，选取造模成功者，通过免疫印迹技术检测NGF及TrkA蛋白表达：采用Morris水迷宫实验检测大鼠空间学习记忆能力。结果（1）MCAO组（脑缺血1h再灌注24h）大鼠NGF的表达低于Sham．MCAO组（P〈0．05），而EX＋MCAO组NGF的表达明显高于MCAO组，但尚未达到Sham—MCAO组水平（P〈0．05）；（2）MCAO组大鼠TrkA蛋白的表达高于Sham．MCAO组（P〈0．05），EX＋MCAO组TrkA的表达则更高于MCAO组（P〈0．05）：（3）Morris水迷宫测试第5天，MCAO组大鼠寻找平台的平均潜伏期[（9．36±1．18）S]与Sham．MCAO组[（4．86±1．52）S]相比明显延长（P〈0．05）；而与MCAO组相比，EX＋MCAO组的潜伏期f（6．02±1．04）S]明显缩短，但仍长于Sham．MCAO组（P〈0．05）。3组大鼠每天的平均游泳速度均差异无统计学意义（P〉0．05）。结论运动训练上调大鼠局灶性脑缺血1h再灌注24h后NGF及其受体TrkA表达．并改善大鼠空间学习记忆能力。
Objective To investigate the effect of exercise preconditioning on the expression of nerve growth factor（NGF） and its receptor TrkA as well as learning-and-memory abilities in rats suffered from focal cerebral ischemia. Methods Thirty-six male SD rats were randomly divided into sham operation group （Sham-MCAO, n= 12） , focal cerebral ischemia-reperfusion group （MCAO, n= 12） and exercise pre- conditioning ＋ cerebral isehemia-reperfusion group （EX＋MCAO, n= 12）. Rats in EX＋MCAO group were placed in the treadmill device and accepted 4 weeks exercise training. Then method of middle cerebral artery occlusion was applied to prepare transient focal cerebral ischemia reperfusion model. Garcia＇ s method was used to assess the neural function in rats. Western blotting was applied to test the expression of NGF and Tr- kA protein in the successfully established experimental MCAO rats. Morris water maze experiment was uti- lized to test the learning-and-memory abilities of the rats. Results （ 1 ） Compared with Sham-MCAO group, the expression of NGF in rats＇ brain tissue was lower in MCAO group （ cerebral ischemia lh reperfusion 24h） （P〈0.05）. The expression of NGF of EX＋MCAO group was higher than that of MCAO group,but still lower than that of Sham-MCAO group （P〈0.05）. （2）The expression of TrkA in rats＇ brain tissue was higher in MCAO group compared with Sham-MCAO group （P〈0.05）.Compared with MCAO group,the expression of TrkA was even higher in EX＋MCAO group （P〈0.05）. （3）On the fifth day in the Morris water maze test,the latency of MCAO group was significantly longer than that of Sham-MCAO group （（ 9.36± 1.18 ）s vs （4.86± 1.52） s, P〈0.05）. However, compared with MCAO group, the latency in EX＋MCAO group （ （ 6.02±1.04） s） was shorter, but still longer than Sham-MCAO group （P〈0.05）. There was no significant difference among the three groups in the average swimming speed （P〉0.05）. Conclusion Exercise preconditioning can up-regu- late the expressions of NGF and TrkA protein, which can also improve the learning-and-memory abilities in rats suffered from focal cerebral ischemia reperfusion injury.
作者
张静
王喜丰
李刚
王岚
沈伟
Zhang Jing Wang Xifeng Li Gang Wang Lan Shen Wei(Department of Internal Neurology, Pu Ai Hospital of Wuhan City, Wuhan 430034, Chin)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2016年第11期966-970,共5页
Chinese Journal of Behavioral Medicine and Brain Science
基金
国家自然科学基金项目（31300882）
武汉市卫计委临床医学科研项目（WX13C19）
