摘要
在低盐水体(盐度12)条件下,利用实验生态学方法采用间歇式流水呼吸仪测定了不同温度(21℃、24℃、27℃、30℃及33℃)、p H(6.5、7.0、7.5、8.0及8.5)及体重(均值:15.64 g、35.80 g、65.67 g和95.93 g)对斜带石斑鱼(Epinephelus coioides)幼鱼耗氧率(MO_2)和排氨率(M_(TAN))的影响。结果表明,温度对斜带石斑鱼幼鱼MO_2和M_(TAN)有显著性影响(P<0.05),随着温度的上升均呈现上升的变化,二者与温度(T)之间的关系均可以用线性函数来拟合(MO_2=6.0826T﹣8.9704,R^2=0.9127;M_(TAN)=0.2248T﹣0.7731,R^2=0.7792)。在实验温度范围,呼吸温度系数Q10和排泄温度系数Q10均值分别为1.51和1.54,而且在水温27℃和30℃时最小,这表明斜带石斑鱼适宜生长的温度范围为27~30℃。p H对斜带石斑鱼幼鱼MO_2和M_(TAN)有显著性影响(P<0.05),随p H上升均呈现先升后降的变化,二者与p H之间的关系均可以用二次函数来拟合(MO_2=﹣15.241Ap H_2+234.98Ap H﹣737.42,R^2=0.7888;M_(TAN)=﹣1.1477Ap H_2+18.073Ap H﹣65.369,R^2=0.7557)。体重对斜带石斑鱼幼鱼MO_2和M_(TAN)有显著性影响(P<0.05),随着体重的增加均呈下降的变化,与体重(W)之间关系均可以用幂函数来拟合(MO_2=310.61 W﹣0.1972,R^2=0.8653;M_(TAN)=9.9167W﹣0.2043,R^2=0.8257),而耗氧量(RO_2)和排氨量(R_(TAN))随体重的增加均呈上升的变化,与体重之间的关系均可以用幂函数来拟合(RO_2=0.3106W 0.8028,R^2=0.9907;R_(TAN)=0.0099W 0.7957,R^2=0.9863)。幼鱼的氧氮比均值在本实验温度范围为25.90,在本实验p H范围其为28.65,在本实验体重范围其为28.19。这提示斜带石斑鱼幼鱼在低盐水体养殖主要以蛋白质和脂肪为能量来源。
Orange-spotted Grouper （Epinephelus coioides） which inhabits subtropical and tropical areas, has become a very popular species of marine teleost currently being cultured in China. Metabolic rate is the most fundamental biological rate as it represents the rate of energy uptake, transformation and allocation. Oxygen consumption is a widely studied indicator of metabolic rate, and measurement of oxygen consumption rate is often used to examine energy utilization and stress in aquatic animals. The aim of the present study is to investigate the effects of temperature （21℃, 24℃, 27℃, 30℃ and 33℃）, pH （6.5, 7.0, 7.5, 8.0 and 8.5） and body wet weight （mean values：15.64 g, 35.80 g, 65.67 g and 95.93 g） on oxygen consumption rate （MO2）, ammonia excretion rate （MTAN） of Orange-spotted Grouper juveniles cultured in low-salt water （salinity 12） by the intermittent flow respirometry system with ecological methods in laboratory. Data were analyzed by using the one-way ANOVA, followed by Ducan test. All analyses were performed with a significance level of P 〈 0.05. The results showed that temperature strongly affected both MO2 and MTAN. The MO2 and MTAN increased significantly （P 〈 0.05） with an increase in temperature from 21℃ to 33℃ （Table 1）. The linear equation between the MO2, MTAN and temperature （T） were established （MO2 = 6.0826T﹣8.9704, R^2 = 0.9127; MTAN = 0.2248T﹣0.7731, R^2 = 0.7792） （Fig. 2）. Over the entire experimental temperature range （21﹣33℃）, the respiration temperature coefficient Q10 and excretion temperature coefficient Q10 were 1.51, 1.54, respectively, and the lowest values were found between 27 and 30℃. The optimal temperature for the juvenile lied between 27 and 30℃ （Table 2）. Both MO2 and MTAN were significantly affected by pH （P 〈 0.05） （Table 1）, being increased first then decreased with pH increase. The relationship between MO2, MTAN and pH was modeled by a quadratic equation （MO2 = ﹣15.241ApH^2 ＋ 234.98ApH ﹣737.42, R^2 = 0.7888; MTAN =﹣1.1477ApH^2 ＋ 18.073ApH﹣65.369, R^2 = 0.7557） （Fig. 2）. Both MO2 and MTAN decreased significantly with increased body wet weight （P 〈 0.05） （Table 1）, and their relationship with body wet weight （W） could be represented by power equations （MO2 = 310.61W ^﹣0.1972, R^2 = 0.8653; MTAN = 9.9167W ^﹣0.2043, R^2 = 0.8257）. The oxygen consumption （RO2） and ammonia excretion （RTAN） increased with increase in body mass, and their relationship with body wet weight was modeled by power equations （RO2 = 0.3106W ^0.8028, R^2 = 0.9907; RTAN = 0.0099W ^0.7957, R^2 = 0.9863）. The averages of oxygen-nitrogen ratio （O：N） at different temperatures, pH values and body wet weights were 25.90, 28.65 and 28.19, respectively （Table 1）, which indicated that Orange-spotted Grouper juvenile cultured in low- salt water utilized protein-lipid dominated metabolism.
出处
《动物学杂志》
CAS
CSCD
北大核心
2016年第6期1038-1048,共11页
Chinese Journal of Zoology
基金
广东高校优秀青年创新人才培养计划项目(No.2013LYM-0038)
广东省海洋渔业科技推广专项项目[No.A2010-08D04
A2010004-016(c)]
广东省海洋经济创新发展区域示范专项(No.GD2012-A02-003)
广东海洋大学科研启动经费(No.E15183)
关键词
斜带石斑鱼
耗氧率
排氨率
低盐水体
Orange-spotted Grouper, Epinephelus coioides
Oxygen consumption rate
Ammonia excretion rate
Low-salt water
