摘要
本研究利用拟南芥两条循环电子传递途径的突变体——NDH途径的突变体crr4、PGR5/PGRL1途径的突变体pgr5以及两条途径的双突变体crr4/pgr5来研究低浓度NaHSO_3在不同循环电子传递途径中的作用机制。结果显示,低浓度NaHSO_3处理增加了光-暗-光转换条件下Ndh H的积累和持续光照条件下PGR5的积累。低浓度NaHSO_3明显地促进了pgr5在光-暗-光转换条件下、暗-光转换条件下和持续光照条件下crr4的循环电子传递速率,表明低浓度NaHSO_3也参与了PGR5途径的运转。与pgr5相比,低浓度的NaHSO_3处理明显提高了持续光照下crr4突变体的跨类囊体膜质子梯度的建立和热耗散能力。根据这些结果我们推测,低浓度NaHSO_3在光下通过促进PGR5蛋白的表达,增加依赖PGR5途径的跨类囊体膜质子梯度和热耗散能力,在光保护中发挥重要的作用。
In this work, we investigated the effect of low concentration of NaHSO3 on PGR5/PGRL1 pathway using Arabidopsis thaliana defective mutants in two pathways of cyclic electron transport around photosystem I （PSI）-cyclic electron flow-NDH pathway defective mutants crr4, PGR5/PGRL1 pathway defective mutant pgrS, and both pathways defective double mutant, crr4/pgr5. Our results showed that the treatment of low con- centration NaHSO3 increased NdhH accumulation under the condition of light-dark-light transition and PGR5 under the continuous illumination conditions. Accordingly, low concentration of NaHSO3 promoted the rate of PSI-cyclic electron flow either in pgr5 mutant under the condition of light-dark-light transition or in crr4 mu- tant under both the light-dark-light transition and continuous illumination, indicating that low concentrations of NaHSO3 participated in the regulation of PGR5-pathway. Compaired with pgrS, low concentration of NaHSO3 significantly increased the formation of proton gradient across the thylakoid membrane and the heat dissipation capacity in crr4 mutant under continuous light. Based on these results we suggest that low concentration NaH- SO3 plays an important role in photo-protection by promoting the expression of PGR5 proteins, increasing the trans-thylakoid membrane proton gradient and heat dissipation capacity through PGR5 pathway.
出处
《植物生理学报》
CAS
CSCD
北大核心
2016年第11期1745-1751,共7页
Plant Physiology Journal
基金
国家自然科学基金(11075400和31470338)
国家重点基础研究发展计划("973"计划)项目(2015CB150104)~~
