摘要
以含氯消毒剂作为防治柑桔黄龙病菌的候选药剂开展药剂筛选试验,将染病芦柑接穗分别浸泡于漂白粉(CH)、二氯异氰尿酸钠(NaDCC)和三氯异氰尿酸泡腾消毒片(TCCA)配制的消毒液12h后再嫁接到健康枳砧上,同时利用定量PCR分析药剂处理前后叶片中脉黄龙病菌含量的变化,以盐酸四环素(TET)为对照药剂,以清水为对照。结果表明,CH处理的药害严重,嫁接成活率低,不适于化学防治。NaDCC和TCCA处理的抑菌效果与处理浓度呈正相关,在有效氯含量为5 000mg/L时病菌减退率最高,平均分别为99.1%和99.5%,该浓度下的相对防效也最佳,平均分别为97.9%和98.8%,与TET的抑制效果相当。NaDCC和TCCA可以作为柑桔黄龙病化学防治的候选药剂。
Huanglongbing（HLB）is the most destructive citrus disease in the world.In this study,scions of ponkan infected with HLB pathogen were dipped into calcium hypochlorite（CH）,or sodium dichloroisocyanurate（NaDCC）,or trichloroisocyanuric acid（TCCA）for 12 hours,then grafted onto healthy trifoliate orange rootstock to screen the disinfectants against HLB.Changes of HLB pathogen in leaves before and after treatment were detected by PCR analysis.TET was used as control chemicals.The results indicated that CH was toxic to citrus plants with low scions surviving rate.The inhibiting effect of NaDCC and TCCA was positively correlated to the concentrations.The concentration of NaDCC and TCCA with the highest therapeuticefficiency（99.1%and 99.5%,respectively）was 5 000mg/L of valid chlorine,which also had the relative therapeutic efficiency of97.9% and 98.8%,respectively.These two disinfectants were highly effective in reducing pathogen as well as tetracycline hydrochloride（TET）.The results suggested that NaDCC and TCCA could be used as potential bactericide to control HLB.
出处
《中国南方果树》
北大核心
2016年第6期6-9,14,共5页
South China Fruits
基金
公益类科研院所专项(2016R1013-14)
福建省农业科学院科技创新团队PI项目(2016-PI37)
国家公益性行业(农业)科研专项(201003067-05)资助
关键词
柑桔黄龙病
含氯消毒剂
病菌含量
相对防效
citrus huanglongbing
disinfectants
pathogen content
relative therapeutic efficiency
