摘要
目的建立RP-HPLC法同时测定复方苦木消炎片（穿心莲和苦木）中5种成分的含有量。方法该药物50%乙醇提取液的分析采用Agilent Eclipse Plus C18色谱柱（250 mm×4.6 mm,5μm）;流动相乙腈（A）-0.1%磷酸（B）,梯度洗脱;体积流量1.0 m L/min;柱温25℃;检测波长254 nm（0~26 min,4-甲氧基-5-羟基铁屎米酮）、225 nm（26~40 min,穿心莲内酯）、205 nm（40~55 min,新穿心莲内酯、去氧穿心莲内酯、脱水穿心莲内酯）。结果4-甲氧基-5-羟基铁屎米酮、穿心莲内酯、新穿心莲内酯、去氧穿心莲内酯、脱水穿心莲内酯分别在0.055~1.378、0.099~2.470、0.045~1.115、0.041~1.030、0.073~1.813μg范围内呈良好的线性关系,平均加样回收率分别为98.6%、99.1%、97.1%、101.0%、97.8%,RSD（n=6）分别为1.3%、0.98%、1.3%、1.6%、1.7%。结论该方法简便快捷,可用于复方苦木消炎片的质量控制。
AIM To establish an RP-HPLC method for the simultaneous content determination of five constituents in Compound Kumu Xiaoyan Tablets（ an anti-inflammatory agent containing Andrographis Herba and Picrasmae Ramulus et Folium）. METHODS The analysis of 50% ethanol extract of this drug was performed on a 25 ℃thermostatic Agilent Eclipse Plus C18column（ 250 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of acetonitrile（ A）-0. 01% phosphate acid（ B） flowing at 1 m L / min in a gradient elution manner,the detection wavelengths were set at 254 nm（ 0- 26 min） for 4-methoxy-5-hydroxycanthin-6-one,225 nm（ 26- 40 min） for andrographolide,and 205 nm（ 40- 55 min） for neoandrographolide,deoxy-andrographolide and dehydroandrographolide. RESULTS 4-Methoxy-5-hydroxycanthin-6-one,andrographolide,neoandrographolide,deoxy-andrographolide and dehydroandrographolide showed good linear relationships within the ranges of 0. 055- 1. 378 μg,0. 099- 2. 470 μg,0. 045- 1. 115 μg,0. 041- 1. 030 μg and 0. 073- 1. 813 μg,whose average recoveries were98. 6%,99. 1%,97. 1%,101. 0% and 97. 8% with the RSDs（ n = 6） of 1. 3%,0. 98%,1. 3%,1. 6% and1. 7%,respectively. CONCLUSION This simple method can be used for the rapid quality control of Compound Kumu Xiaoyan Tablets.
出处
《中成药》
CAS
CSCD
北大核心
2016年第12期2590-2593,共4页
Chinese Traditional Patent Medicine
