摘要
目的探讨结直肠癌患者血清中miR135a-5p的表达水平及其辅助诊断价值。方法收集60例结直肠癌患者、40例结直肠息肉患者及50例健康对照者血清样本，分别检测其血清miR135a-5p、癌胚抗原（CEA）及糖类抗原199（CA199）含量，分析结直肠癌患者血清miR135a-5p水平与其临床病理特征之间的关系，以及结直肠癌患者血清miR135a-5p水平与CEA和CA199的关系。应用受试者工作特征曲线和曲线下面积（AUC）评估miR135a-5p、CEA和CA199作为诊断指标的诊断效能。结果结直肠癌患者、结直肠息肉患者和健康对照者血清miR135a-5p的相对表达量分别为2．451、0．946和0．949。结直肠癌患者血清miR135a-5p的相对表达量明显高于结直肠息肉患者和健康对照者，差异均有统计学意义（均P〈0．001）。结直肠癌患者血清miR135a-5p的相对表达量与肿瘤的分化程度和肿瘤分期有关（均P〈0．05）。结直肠癌患者血清miR135a-5p的相对表达量与CEA和CA199的表达均无关（均P〉0．05）。以结直肠息肉患者为标准，血清miR135a-5p诊断结直肠癌的AUC为0．832;以健康对照者为标准，血清miR135a-5p诊断结直肠癌的AUC为0．875。结论结直肠癌患者血清miR135a-5p的含量高于结直肠息肉患者和健康对照者。血清miR135a-5p可能是一个重要的诊断结直肠癌的生物学指标。
Objective To explore the expression level of serum miR135a-5p and its diagnostic value in colorectal cancer （CRC）. Methods Serum samples were randomly collected from 60 primary CRC patients, 40 patients with intestinal polyps and 50 healthy controls, and the serum concentrations of miR135a-5p, CEA and CA199 were detected. The relationships between serum miR135a-5p level and clinicopathological parameters were analyzed by Mann-Whitney U test. The correlation of serum miR135a-5p level and serum concentrations of CEA or CA199 was analyzed by Pearson＇ s correlation test. Receiver operating characteristic （ROC） curves and area under the curve （AUC） were used to evaluate the diagnostic efficacy of miR135a-5p, CEA and CA199 as diagnostic indicators. Results The serum level of miR135a-5p in CRC patient was 2.451 （1.107, 4.413）, significantly higher than 0.946 （0.401, 1.942） in the patients with intestinal polyps and 0.949 （0. 194, 1.415） in the healthy controls （U = 351.0 and U = 313.0, respectively, P〈 0. 001 ）. The serum level of miR135a-5p in CRC patients was associated with both histological differentiation and clinical stage （P〈 0.05 for both）, however, not correlated with the serum concentration of CEA （r^2= 0.023, P = 0.293） or CA199 （r^2= 0.067,P = 0.068）. The AUC of serum miR135a-5p level in CRC patients was 0.832 （0.730-0.930） when compared to the patients with intestinal polyps and was 0.875 （0.800-0.950） when compared with the healthy controls. Conclusions The serum level of miR135a-5p in CRC patients is significantly higher than that in patients with intestinal polyps and heahhy controls, and might be an important diagnostic marker of CRC.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2016年第12期909-914,共6页
Chinese Journal of Oncology
基金
国家自然科学基金（81271920、81301498）
江苏省“科技兴卫工程”创新团队项目（LJ201133）
