摘要
以超强抗寒性冬油菜品种陇油7号分别与抗寒性品种延油2号和耐寒性品种天油2号的杂交后代(F1、F2、BC1)为研究对象,对各世代越冬率进行统计并结合亲本及杂交后代的CAT、POD、SOD酶活性变化研究,分析它们之间的抗寒性关系。结果表明:不同抗寒性品种杂交后代的越冬率均介于2个亲本之间,但不同世代越冬率存在较大差异;母本的抗寒性越强,在F1代中,后代的越冬率越高,F1代的自交后代(F2)越冬率比F1代的低;在BC1中,后代的越冬率变化范围较大,既有低于F2代自交后代的杂交组合(天2×陇7)×天2,也有高于F1代的杂交组合(陇7×天2)×陇7,以抗寒性强的品种作轮回亲本可以使回交一代的越冬率明显升高。低温胁迫后,2个群体不同世代的保护酶活性均增加,在群体1中,F1代CAT平均值从降温后比降温前升高了,F2从8.41 U·g-1到15.72 U·g-1,BC1从10.62 U·g-1到19.20 U·g-1。对越冬率与保护性酶进行回归分析,回归方程为Y=1.208+2.698X1+1.154X2+0.163X3,可得出越冬率与CAT酶、POD酶、SOD酶活性的关系呈极显著正相关,说明保护性酶活性越强,越冬率越高,抗寒性也就越好。
Taken the filial generations（F1,F2,BC1） of the ulterstrong cold resistance variety Longyou 7 with cold resistance variety Yanyou2 and cold tolerance variety Tianyou 2 as tested materials,their each generation wintering rates were estimated,combining with their parents and filial generations CAT,POD,SOD enzyme activity change,and their cold resistance relationship between them were analyzed.The results showed that wintering rates in filial generations of different cold resistance winter turnip rape between the two parent,but there were large differences between different generations; In F1 generation,the stronger the cold resistance of female parent was,the higher the wintering rate of offspring was,and F2 generation of selfing generations of wintering rate was lower than that of the F1generation; In BC1 generations,the wintering rate range was larger,there were lower than that of F2 generation of selfing generations of hybrid combinations（Tianyou 2 × Longyou 7） × Tian you 2 and higher than that of F1 generation hybrid combination（Longyou 7 ×Tianyou） × Long you 7,and the variety with ultrastrong cold resistance as recurrent parent could make backcross generation wintering rate increase significantly.After low temperature stress,the protective enzyme activity of different generations of two groups in-creased to some extent,in group 1,the F1 generation CAT average from before cool to after cooling was increased,F2 from 8.41 to 15.72 U·g^-1,and BC1 from 10.62 to 19.20 U·g^-1.By regression analysis for wintering rates and protective enzyme,their regression equation was Y = 1.208 ＋ 2.698X1＋ 1.154X2＋ 0.163X3,and the relationship of wintering rates with CAT,POD and SOD were significantly positive,indicating that the stronger the protective enzyme activity was,the higher the rate was,and the better the cold resistance was.
出处
《西南农业学报》
CSCD
北大核心
2016年第11期2529-2535,共7页
Southwest China Journal of Agricultural Sciences
基金
国家现代农业产业技术体系“北方寒旱区冬油菜育种岗位科学家”(CARS-13)
国家自然科学基金“白菜型油菜抗寒评价指标体系及数学模型构建”(31460356)
国家自然科学基金(31560397)
973计划-油菜高产油量形成的分子生物学机制(2015CB150206)
国家863计划专项“强优势油菜杂交种的创制与应用”(2011AA10A104)
国家农业科技成果转化项目(2014G10000317)
甘肃省自然科学基金(145RJZG050)
甘肃省高等学校科研项目(2014B-049)
关键词
冬油菜
白菜型
越冬率
保护酶活性
抗寒性
杂交后代
Winter rapeseed
Brassica rapa L.
Wintering rate
Protective enzyme activity
Cold resistance
Filial generations
