摘要
目的:探讨阿司匹林对于宫颈癌Hela细胞的凋亡诱导作用以及增殖抑制的相关机制。方法:通过离体培养宫颈癌Hela细胞,以不同剂量的阿司匹林对其增殖过程进行干预,利用Annexin V-FITC/PI凋亡试剂盒检测Hela细胞的凋亡情况。同时,采用Western-blot法检测NF-κB p65、Bcl-2以及Bax蛋白的表达情况。最后,通过碘化丙啶(PI)染色测定宫颈癌Hela细胞周期分布情况。结果:阿司匹林1.0、5.0、10.0 mmol/L剂量组能够显著增加宫颈癌Hela细胞的凋亡率,其S期、G_2/M期细胞所占比例明显减少,同时G_0、G_1期细胞分布明显提高。NF-κB p65蛋白、Bcl-2蛋白表达水平显著降低,其下降程度与所接受阿司匹林剂量有明显相关性,同时Bax蛋白表达水平提高。结论:阿司匹林能够通过抑制NF-κB p65、Bcl-2蛋白表达水平并提高Bax蛋白表达水平,干预相关细胞周期分布最终诱导宫颈癌Hela细胞的凋亡,其诱导机制的强弱程度呈现明显的剂量相关性。
AIM： To study the mechanism of aspirin induced apoptosis and inhibiting proliferation of cervical cancer Hela cells. METHODS： Diverse doses of aspirin were used in cervical cancer Hela cells cultured in vitro. The apoptosis rate of Hela cells by Annexin V-FITC/PI was calculated. Meanwhile, Western-blot was taken into study to analyze the expression of NF-κB p65, Bcl-2 and Bax pro- tein. The cell cycle of Hela cells was analyzed by propidium iodide staining. RESULTS：Aspirin reduced the apoptosis rate of Hela cells by dose of 1.0, 5.0 and 10.0 mmol/L respectively. More cells were detected in period Go and G1 while that were less seen in period S and G2/M. At last the expres- sion of NF-κB p65 protein and Bcl-2 protein were cut down yet the Bax protein was raised. CONCLUSION： Aspirin can restrain the expression of NF-κB p65, Bcl-2 protein and promote the expression of Bax protein by its different doses, which lead a higher apoptosis rate in Hela ceils.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2016年第11期1263-1266,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
安徽省高校省级自然科学研究项目(KJ2015B096by)
