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人参二醇的微生物转化及抗HSC-T6细胞活性研究 被引量:1

Microbial Transformation of 20(R)-Panaxadiol and Their Activity Against HSC-T6 Cells
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摘要 研究了20(R)-人参二醇衍生物转化及其抗HSC-T6细胞活性。利用微生物刺囊毛霉Mucorspinosus AS 3.3450对20(R)-人参二醇进行转化,采用硅胶柱色谱、制备型高效液相色谱技术分离、纯化产物,并通过波谱数据分析确认了产物结构。采用噻唑蓝(MTT)法测试产物抗HSC-T6肝星状细胞活性。共分离鉴定出了5个化合物:3-羰基-20(R)-人参二醇(1),3-羰基-7β,24α-二羟基-20(R)-人参二醇(2),3-羰基-7β,24β-二羟基-20(R)-人参二醇(3),3-羰基-15α,24α-二羟基-20(R)-人参二醇(4),3-羰基-15α,24β-二羟基-20(R)-人参二醇(5)。MTT结果显示,化合物1~3抑制HSC-T6细胞增值的作用强于20(R)-人参二醇。 To investigate the derivatives of 20(R)-panaxadiol and their activities against HSC-T6 cells,20(R)-panaxadiol was treated by Mucorspinosus AS 3.3450.All products were isolated by silica gel chromatography and purified by preparative HPLC.On the basis of spectral data,structures were elucidated.The activity against HSC-T6 cells was measured by MTT method.Five biotransformation products were obtained and identified as 3-oxo-20(R)-panaxadiol(1),3-oxo-7β,24α-dihydroyl-20(R)-panaxadiol(2),3-oxo-7β,24β-dihydroyl-20(R)-panaxadiol(3),3-oxo-15α,24α-dihydroyl-20(R)-panaxadiol(4),3-oxo-15α,24β-dihydroyl-20(R)-panaxadiol(5).MTT assay revealed that compounds 1-3 showed higher inhibitory activity against HSC-T6 cells than 20(R)-panaxadiol.
机构地区 南通大学药学院
出处 《常州大学学报(自然科学版)》 CAS 2016年第6期23-27,共5页 Journal of Changzhou University:Natural Science Edition
基金 江苏省高等学校大学生创新创业训练计划项目(201610304075Y) 南通市科技项目(MS12015044)
关键词 人参二醇 微生物转化 HSC-T6细胞 抗肝纤维化 20(R)-panaxadiol microbial transformation HSC-T6 anti-liver fibrotic
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