摘要
以壳聚糖(CS)为基体、以1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐(EDC.HCl)为缩合剂、N-羟基-丁二酰亚胺(NHS)为偶联剂,将L-精氨酸固载到CS大分子上,L-精氨酸接枝壳聚糖CS-L-Arginine(CA),通过单因素实验优化接枝工艺,制备出一种天然高分子基重金属螯合材料。应用元素分析、红外光谱(FT-IR)、拉曼光谱(RAM)和X-射线衍射(XRD)等手段表征了产物的结构组成,研究了接枝机理。用坂口反应并结合凯氏定氮法测定了产物的接枝率GR。结果表明,当反应物物质的量比为n CS:n Arg:n EDC=1:1:1、n EDC:n NHS=3:1、反应时间12 h、体系的pH值=5时,接枝率(GR)可达16.85%。调整反应物的投入比,可制备出接枝率不同的接枝产物。吸附实验结果表明,与CS相比,CA(GR=16.85%)使高浓度溶液中的Cu^(2+)、Ni^(2+)离子的去除率显著提高,CA的抗菌性比CS也显著增强,GR为16.85%的CA几乎完全抑制了大肠杆菌和金黄色葡萄球菌的生长。
For improving the heavy metal ion chelating ability of chelatingheavy metal ions and the microbiological stability of chitosan （CS）, L-arginine （L-Arg） was immobilized on CS molecules, in pres- ence of condensing agent 1-（3-Dimethylaminopropyl）-3-ethylcarbodiimide hydrochloride （EDC.HCI） and coupling reagent N-Hydroxysuccinimide （NHS）, using chitosan as a matrix, a new kind of natural-macro- molecular-based heavy metal ion chelating material, L-arginine GRafted Chitosan （CA）of chelating heavy metal ions, was prepared by grafting L-arginine （L-Arg） onto CS molecules, with chitosan as raw mate-ri- al, 1-（3-Dimethylaminopropyl）-3-ethylcarbodiimide hydrochloride （EDC.HCI） ascondensing agent and N- Hydroxysuccinimide （NHS） coupling reagent. The GRafting mechanism was deeply discussed and the GRafted conditions were optimized by signal factor experiments. The structure and composition of the product were characterized by elemental analysis, infrared spectrum （FT-IR）, Raman spectra （RAM） and x-ray diffraction （XRD）. Then, the processing parameters were optimized via experiments with single fac- tor. The results show that a grafting ratio （GR） of 16.85% may be acquired when the for the following pa- rameters： molar ratios of reagents CS：Arg：EDC =1：1：1 and EDC：NHS =3：1, reaction time 12 h and pH=5, the GRating ratio （GR） was up to 16.85%. By adjusting the ratio of reactants, products with different GR levels could be obtained. The results of adsorption experiments showed CA （GR=16.85%） had higher re- movalefficiency of high-concentrated Cu2＋ and Ni2＋ ions than CS.CA also possessedhigher antibacterial property compared with CS, whilst the CA with 16.85% GRof was up to 16.85%, it can almost completely inhibited the growth of colibacillus and staphylococcus aureus.
出处
《材料研究学报》
EI
CAS
CSCD
北大核心
2016年第11期825-833,共9页
Chinese Journal of Materials Research
基金
国家自然科学基金5150030497
国家级现代农业产业技术体系专项CARS-44-D资助项目~~
关键词
有机高分子材料
氨基酸改性壳聚糖
接枝
金属离子吸附
抗菌性
organic polymer materials, amino acid-modified Chitosan, GRaft, metal ion absorption antibacterial property
