摘要
目的:探讨富集AT序列的特异性结合蛋白1(special AT rich sequence binding protein-1,SATB1)与核糖核苷酸还原酶(ribonucleotide reductase,RR)小亚基M2(RRM2)在非霍奇金淋巴瘤(non-Hodgkin lymphoma,NHL)恶性进展中的相互作用机制。方法:收集NHL及慢性淋巴结炎患者各42例。Western印迹检测NHL及慢性淋巴结炎患者颈部淋巴结冰冻组织中的SATB1和RRM2的蛋白表达水平;RT-PCR检测过表达和干扰SATB1后RRM2 m RNA的表达水平的变化;MTT及Ed U渗入法检测过表达SATB1及同时干扰RRM2后对细胞生长增殖的影响。Transwell法检测过表达SATB1及同时干扰RRM2后对细胞迁移和侵袭的影响。结果:NHL患者淋巴结冰冻组织中SATB1及RRM2表达水平明显高于慢性淋巴结炎患者,且两者的表达水平呈正相关。过表达SATB1后,RRM2蛋白和m RNA的表达水平上调;干扰SATB1后,RRM2蛋白和m RNA的表达水平下降。MTT及Ed U检测结果表明过表达SATB1后NHL细胞生长增殖能力增加,而同时干扰RRM2后生长增殖能力受到抑制。Transwell结果表明过表达SATB1后NHL细胞迁移侵袭能力增加,而同时干扰RRM2后迁移侵袭能力受到抑制。结论:SATB1在NHL中高表达,其可能通过上调RRM2基因的表达促进NHL的生长增殖、迁移和侵袭。
Objective： To explore the role of the special AT rich sequence binding protein-1（SATB1） and ribonucleotide reductase M2（RRM2） in enhancing malignant progression of non-Hodgkinlymphoma（NHL）. Methods： A total of 42 NHL and 42 chronic lymphadenitis patients were recruited. The protein expressions of SATB1 and RRM2 in cervical lymph nodes were determined by Western blot. After overexpression of SATB1, si SATB1 or si RRM2, the m RNA levels of SATB1 and RRM2 in cells were analyzed via RT-PCR, the cell proliferation was evaluated via MTT and EdU assays, while the migration and invasion of cells were assessed by transwell assays.Results： Compared with chronic lymphadenitis, the expressions of SATB1 and RRM2 in NHL patients were up-regulated. There was positive correlation between SATB1 and RRM2 in NHL patients. RRM2 mR NA level was up-regulated after transfection of SATB1 and down-regulated after transfection of si SATB1. Overexpression of SATB1 increased tumor cell proliferation, migration and invasion, while knockdown of RRM2 reversed those phenomena.Conclusion： SATB1 functions as an oncogene and promotes tumor cell proliferation, migration and invasion by up-regulation of RRM2 in NHL.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2016年第11期1155-1162,共8页
Journal of Central South University ：Medical Science
基金
国家自然科学基金(8130165)~~
