摘要
试验研究了Lactobacillus plantarum P-8与Saccharomyces cerevisiae QH2-2高密度混合发酵培养基、发酵条件优化及其冻干菌粉的制备。通过对氮源、发酵温度以及接菌工艺的优化证实以大豆蛋白粉为氮源,30℃同时接菌能获得更高的L.plantarum P-8和S.cerevisiae QH2-2活菌数（分别为5.98×10^9 CFU/mL和3.15×10^7 CFU/mL）,较优化前分别提高了2.11倍和3.09倍。在此基础上,上发酵罐对发酵条件进行优化证实同时接菌（L.plantarum P-8 6%和S.cerevisiae QH2-2 1%）,30℃下前7 h通空气,7 h后不通气条件下,L.plantarum P-8活菌数可达到1.66×10^10 CFU/mL,S.cerevisiae QH2-2活菌数可达到6.21×10^7 CFU/mL,较上发酵罐前分别提高了2.77倍和1.97倍。L.plantarum P-8和S.cerevisiae QH2-2混合发酵液经冷冻干燥获得L.plantarum P-8的活菌数2.91×10^11 CFU/g,S.cerevisiae QH2-2的活菌数1.10×10^9 CFU/g。上述菌种的混合发酵为益生菌发酵剂和微生态制剂的制备提供了参考。
The high density cultivation of Lactobacillus plantarum P-8 fermented with Saccharomyces cerevisiae QH2- 2 was studied by optimizing culture condition and preparing freeze-drying powder. The results showed that soy protein powder was used as nitrogen source, inoculated simultaneously at 30 ℃ with highly viable counts of L. plantarum P-8 and S. cerevisiae QH2-2 （5.98× 10^9 CFU/mL and 3.15× 10^7 CFU/mL, respectively）, which were 2.11 and 3.09 times as many as before, respectively. And on this basis, it was proved that inoculated （L. plantarum P-8 6% and S. cerevisiae QH2-2 1%） simultaneously at 30 ℃, ventilated 7 h and stopped ventilating 7 h later with the most highly viable counts ofL plantarum P-8 and S. cerevisiae QH2-2 in fermentation tank. The viable counts were 1.66× 10^10 CFU/mL and 6.21 × 10^7 CFU/mL which were 2.77 and 1.97 times as many as before. The viable counts of freeze-drying powder were 2.91 × 10^11 CFU/g of L. plantarum P-8 and 1.10×10^9 CFU/g of S. cerevisiae QH2-2, L. plantarum P-8 fermented with S. cerevisiae QH2-2 provided a reference for preparing the probiotic starters and microecologics.
出处
《食品工业》
CAS
北大核心
2016年第12期34-38,共5页
The Food Industry
关键词
乳酸菌
酿酒酵母
培养基优化
混合发酵
冷冻干燥
Lactobacillus plantarum
Saccharomyces cerevisiae
medium optimization
mixed fermentation
freeze-drying
