摘要
[目的]对湖北咸宁地区山坡土样中分离得到的1株放线菌HBERC-31270的发酵提取物进行快速鉴定及活性评价。[方法]对HBERC-31270进行发酵与提取,并对发酵提取物进行生物活性测定、高效液相色谱-质谱（HPLC-MS）、紫外吸收光谱（UV）分析及活性评价。[结果]提取物高效液相色谱（HPLC）制备组分活性测定结果表明,其活性部位在14-21 min。根据HPLC-MS及UV确定其主要成分为一个小分子化合物。通过对其质谱数据进行分析确定了分子离子峰,经天然产物数据库比对及文献调研,确定其主要活性成分为9-甲基链米酮。盆栽试验结果表明,该菌对多种作物真菌性病害具有较好的防治效果。[结论]试验结果为放线菌HBERC-31270的进一步研究提供了理论依据。
[Objective] Rapid identification and activity evaluation were carried out for the fermentation extracts of a strain HBERC-31270 which was isolated from a soil sample collected in Xianning,Hubei province.[Method] The strain HBERC-31270 was fermented and extracted,and bio-activity determination,HPLC-MS,UV spectrum analysis and activity evaluation were carried out for the fermentation extracts.[Result] It was de-termined by components bioassay that the active compounds were in between 14-21 min in HPLC profile,and the compounds were identified as 9-methylstreptimidone on the basis of MS,UV absorption spectrum and natural product dictionary.It was found by pot test that the fermentation broth was very effective against a variety of fungal diseases.[Conclusion] The results provide theoretical basis for the further study of actinomyce-tes HBERC-31270 .
出处
《安徽农业科学》
CAS
2016年第33期9-11,14,共4页
Journal of Anhui Agricultural Sciences
基金
湖北省农业科技创新中心项目(2016-620-000-001-039)
关键词
天然产物
农药活性
放线菌
9-甲基链米酮
Natural products
Pesticide activity
Actinomycetes
9-methylstreptimidone
