摘要
轮状病毒是引起幼龄动物和儿童病毒性腹泻的主要病原,轮状病毒的分离鉴定为该病的流行病学调查和分子生物学特性研究奠定了基础。本研究采集北京某猪场腹泻发病仔猪肠内容物,将其接种MA104细胞,分离得到一株能产生明显细胞病变的病毒。对分离毒株进行胶体金、实时荧光定量RT-PCR和免疫荧光等方法鉴定,并对分离毒株的VP4、VP6和VP7基因进行测序及序列分析。结果表明,分离毒株为猪A群轮状病毒。按照A群轮状病毒的最新分类方法,确定该分离株VP4、VP6和VP7基因的基因型分别为P[13]型、I5型和G11型。因此,将该分离株命名为Rotavirus A pig/China/BJ/2015/G11P[13]。
Rotavirus infections are a major cause of viral diarrheas in young animals and children. Isolation and identification of rotavirus make a contribution to epidemiological survey and molecu-lar biology study.A strain of porcine rotavirus was isolated in MA104 cell cultures from the intes-tinal contents of piglets with diarrhea in Beijing.The virus was identified to be porcine rotavirus by immunochromatography strip test,Real-time RT-PCR,immunofluorescence test and sequen-cing analysis.According to the sequence analysis,the virus was classified as group A porcine ro-tavirus,the genotype ofVP 4,VP 6 and VP 7 genes belonged to P[13],I5 and G1 1,respectively. The virus was designated Rotavirus A pig/China/BJ/2015/G1 1P[13].
出处
《中国畜牧兽医》
CAS
北大核心
2016年第12期3306-3313,共8页
China Animal Husbandry & Veterinary Medicine
基金
科技部科技基础性工作专项--动物及动物产品质量安全检测标准物质研制(2013FY113300-6)
关键词
猪轮状病毒
分离
鉴定
porcine rotavirus
isolation
identification
