摘要
目的研究羊栖菜多糖(SFPS)对亲本果蝇生育能力的影响及其作用机制。方法采用85℃热水煮提、乙醇沉淀、氯化钙去除褐藻胶等多种工序提取SFPS;以黑腹果蝇为模式生物,分别以对照(0%)、低(0.033%)、中(0.167%)、高(0.500%)4个浓度持续喂食15 d,通过检测子代数量、体质量、每天羽化量评估不同浓度SFPS对果蝇生育能力的影响;荧光定量PCR(qRT-PCR)技术检测亲本果蝇生育和发育相关基因Vasa、Achi、Figla和子代果蝇mTOR的表达。结果与对照组比较,喂食SFPS能显著性地提高雌蝇和雄蝇的生育力,并且对其子代果蝇体质量、羽化率和雌雄比例也有后续的促进作用,低和中浓度SFPS分别对母本和父本果蝇的生育力促进效果最优;持续喂食SFPS后,亲本果蝇生殖发育相关基因在m RNA水平上显著提高;亲本喂食低、高浓度SFPS后,子代果蝇mTOR表达水平普遍升高,子代雌蝇变化更为显著。结论 SFPS通过调控果蝇生育相关基因的表达,提高亲本果蝇的生育能力,对子代果蝇的生长发育也有显著促进作用。
Objective To estimate effects and possible underlying mechanisms of Sargassum fusiforme polysaccharides （SFPS） on fertility and reproduction, which were oral administered to Drosophila melanogaster. Methods SFPS applied in the following experiments was extracted using 85 hot boiled extraction, purified by ethanol precipitation, and removed alginates by calcium chloride. D. melanogaster were applied as model and fed with four kinds of concentration of SFPS namely control （0%）, low （0.033%）, medium （0.167%）, and high （0.500%）, for 15 d. Effects of SFPS on fertility were assessed by detecting the number of offspring, body weight, and daily eclosion. Meanwhile, we also determined the expression patterns of fertility and development related genes, such as Vasa, Achi, Figla （parental generation）, and mTOR （filial generation） in different SFPS concentration groups. Results Compared with control group, fertility of flies was significantly enhanced by feeding with SFPS. Moreover, effect on fertility also further facilitates weights, eclosion rates, and gender ratios of offspring flies. However, fertility of father and mother flies performed different dose dependent patterns, and performed best by feeding with low and medium doses of SFPS respectively. The fertility and development related genes mRNA level significantly increased after continuous feeding with SFPS. After parental feeding of low and high concentration ofSFPS, mTOR expression level of offspring increased significantly, female offspring changed more significantly.Conclusion SFPS promotes fertility of D.melanogaster,and which may through upregulating the expression patterns of fertility relatedgenes. Meanwhile, SFPS also obviously promotes development of offspring, when parents are feeding with SFPS.
出处
《药物评价研究》
CAS
2016年第6期925-931,共7页
Drug Evaluation Research
基金
国家自然科学基金资助项目(31470430
31200266)
国家星火计划重点项目(2015GA700005)
浙江省公益性应用研究计划(2017C32103)
温州市公益性农业科技项目(N20150034)
浙江省新苗人才计划(KZS1512066)
