摘要
目的通过构建兔颈动脉粥样硬化(AS)模型,观察全反式维甲酸(ATRA)对AS斑块中转化生长因子-β1(TGF-β1)、兔白介素-6(IL-6)、兔白介素-10(IL-10)和兔白介素-17(IL-17)的表达水平,探讨ATRA的抗AS作用及其机制。方法选取34只纯种雄性新西兰白兔随机分为正常组、模型组、他汀组、ATRA组。正常组给予基础饲料,其余各组给予高脂饲料。实验4周后他汀组予辛伐他汀2 mg/(kg·d)灌胃给药,ATRA组予ATRA 20 mg/d灌胃给药。12周末处死动物,取颈动脉组织观察AS斑块形态及炎症反应情况,并测定血清中血脂、TGF-β、IL-6、IL-10、IL-17的水平。结果模型组颈动脉可见AS斑块形成,内膜断裂。他汀组及ATRA组内皮细胞形态和内膜尚完整。血清中TGF-β1和IL-10水平结果,ATRA组分别为(3 059.49±599.75)ng/L和(46.83±4.56)ng/L,他汀组分别为(2 709.48±601.44)ng/L和(43.13±4.07)ng/L,均比模型组的(2 039.16±638.07)ng/L和(36.39±7.34)ng/L显著增高(P<0.05)。血清中IL-6和IL-17水平结果,ATRA组分别为(64.92±6.64)ng/L和(1 474.88±140.55)ng/L,他汀组分别为(69.54±8.84)ng/L和(1 518.58±136.69)ng/L,均比模型组的(110.00±7.26)ng/L和(2 002.66±195.57)ng/L显著降低(P<0.05)。结论 ATRA具有抗AS的作用,其机制可能与ATRA的炎症免疫调节作用有关。
Objective To investigate the effect and mechanism of all-trans retinoic acid（ ATRA） on the development of atherosclerotic plaque and the expression of TGF-β1,IL-6,IL-10 and IL-17 on the rabbit carotid atherosclerosis model. Method 34 male New Zealand rabbits were randomly divided into four groups,including the normal group,the model group,statin group and ATRA group. The normal group was given basic feed,and other groups were fed with high fat diet. After 4 weeks,rabbits were treated intragastrically with simvastatin D at 2 mg / kg per day or ATRA at 20 g / day. The atherosclerotic plaque and inflammatory response in carotid artery were examined at the end of 12 weeks. Meanwhile,the serum levels of lipids,TGF-β1,IL-6,IL-10 and IL-17 were measured. Result The AS plaque and broken intima were observed in the model groups. The endothelial cell morphology and endometrium were intact in statin- and ATRA-treated groups. Compared with the model group,the levels of TGF-β1 and IL-10 were significantly increased in ATRA group（ 3 059. 49 ± 599. 75） and（ 46. 83 ± 4. 56） ng / L and statin group（ 2 709. 48 ±601.44） and（ 43. 13 ± 4. 07） ng / L. By contrast,the levels of IL-6 and IL-17 were significantly lowered in ATRA group（ 64.92± 6.64） and（ 1 474.88± 140.55） ng / L and statin group（ 69.54± 8.84） and（ 1 518.58± 136.69） ng / L. Conclusion ATRA ameliorates the development of atherosclerosis in rabbit model,which may be related to reduction of the inflammatory response.
作者
彭忠兴
李翔
刁胜朋
黄叶青
程静
刘爱群
危智盛
余青云
洪铭范
PENG Zhongxing LI Xiang DIAO Shengpeng HUANG Yeqing CHENG Jing LIU Aiqun WEI Zhisheng YU Qingyun HONG Mingfan(Department of Neurology, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China)
出处
《广东药学院学报》
CAS
2016年第5期639-642,共4页
Academic Journal of Guangdong College of Pharmacy
基金
广东药学院科技处-附属第一医院联合自然科学培育基金项目(GYFYLH201322)
