摘要
目的建立简单、准确测定水果中7种有机磷农药残留的分析方法。方法取水果样品以丙酮为提取剂经加速溶剂萃取装置(ASE)萃取,提取液无水硫酸钠除水后,在线定量浓缩(EVA),再经全自动凝胶渗透色谱仪(GPC)净化,净化液在线浓缩定容后采用气相色谱-质谱法同时进行分离和定性,外标法定量检测。结果该实验条件下,7种有机磷化合物的线性关系良好,相关系数均>0.999;样品加标的平均回收率为80%~118%,相对标准偏差均<5.0%,该方法的检测限为0.012μg/L^0.084μg/L。结论该方法的提取效率高,稳定性好,准确灵敏,大量实际样品的检测结果表明,此方法适于水果中农药残留检测实际工作的需要。
Objective To establish a method for the simple and rapid detection of 7 organophosphorous pesticide residuals in fruits. Methods The target analytes were extracted with the solution of acetone by speeding,using accelerated solvent extraction（ ASE）,dehydrated with anhydrous sodium sulfate and concentrated under vacuum,then purified using gel permeation chromatography（ GPC）,qualitatively and quantitatively detected by gas chromatography mass spectrometry. Results This method has higher linearity,the correlation coefficient（ r） was greater than 0. 999,the average recovery rate was within 80%-118%,the relative standard deviation（ RSD） was less than 5. 0%,and the detection limit of this method was within 0. 012 μg/L-0. 084 μg/L. Conclusion This method is sensitive,accurate and simple with high efficiency,which is suitable for the determination of pesticide residuals in fruits.
作者
陈平
陆卫明
CHEN Ping LU Wei -ming(Changshu Municipal Center for Disease Control and Prevention, Changshu, Jiangsu 215500, China)
出处
《中国卫生检验杂志》
CAS
2016年第23期3361-3363,共3页
Chinese Journal of Health Laboratory Technology
关键词
加速溶剂萃取
凝胶渗透色谱
气相色谱-质谱
有机磷农药
水果
残留测定
Accelerated solvent extraction
Gel permeation chromatography
Gas chromatography-mass spectrometry
Pesticide
Fruits
Residue determination
