摘要
本研究以魔芋不同外植体为实验材料,建立了魔芋离体培养快速繁殖的再生体系,为以后魔芋基因工程进行品质改良奠定了基础。以不同基因型魔芋为实验材料,研究魔芋不同外植体在不同的培养基中诱导不定芽再生的频率。魔芋不同基因型外植体在含适宜6BA、NAA等激素的培养基中形成愈伤组织的难易程度不同,愈伤组织诱导形成不定芽的能力强弱也不一样;不同基因型魔芋外植体的不定芽诱导率最高的是块茎>幼叶>茎段;对魔芋的块茎、茎段和幼叶来说最适合的培养基配方是MS+6-BA 2.5 mg/L+NAA 0.05 mg/L,不同培养基诱导不定芽的能力是块茎>茎段>幼叶,其中,魔芋块茎和茎段的再生能力较强。最合适的生根培养基是MS+1.0 NAA。本研究成功的建立了魔芋不同外植体离体培养再生途径,并获得再生植株。
Amorphophallus rivieri as explants was studied for rapid propagation through tissue culture.The method we established will be useful for genetic engineering.We investigated the effect of media components on the induction of shoot regeneration for different types of explants in two genotypes of Amorphophallus rivieri.Forming the callus for different genotypes explants cultured on media containing suitable 1-naphthaleneacetic acid(NAA)and 6-benzylaminopurine(6-BA) concentrations was easy,but shoot regeneration from the callus proved difficult.The frequency of shoot regeneration from konjac's different types of explants for all the genotypes were in turn as follows:Tuber>Yong leaves>Stem.The best culture medium for the konjac's explants is MS+6-BA 2.5 mg/L+NAA0.05 mg/L.The ability of inducing shoot regeneration for different explants of Amorphophallus rivieri was very different,and the regenerated frequency of Tuber and stem was the highest.Shooting culture was on MS medium supplemented with NAA 1.0 mg/L.These results indicate that we have successfully established the plant regeneration system of Amorphophallus rivieri,and we have successfully obtained the regenerated plant.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第3期699-703,共5页
Molecular Plant Breeding
基金
贵州省自然科学基金项目(黔科合J字[2013]2246号)
贵州师范学院博士启动基金项目(12BS028)共同资助
关键词
魔芋
外植体
组织培养
再生体系
Amorphophallus rivieri
Explants
Tissue culture
Regeneration
