摘要
目的研究布鲁氏菌病患者血清中可溶性T细胞免疫球蛋白黏蛋白分子3(s Tim-3)与其配体半乳糖凝集素-9(Galectin-9)以及相关细胞因子IFN-γ和IL-4的变化。方法采用酶联免疫吸附实验(ELISA)检测布鲁氏菌病患者血清中s Tim-3和Galectin-9的表达,流式液相多重蛋白定量技术(CBA)检测IFN-γ和IL-4的变化,Pearson法分析布鲁氏菌病患者血清中s Tim-3、IFN-γ及IL-4的相关性。结果与正常对照组相比,布鲁氏菌病患者组血清中的s Tim-3和Galectin-9及IL-4的表达均增加(P<0.05),IFN-γ表达减少(P<0.01),并且布鲁氏菌病患者血清中s Tim-3的表达与IL-4呈正相关(r=0.87,P<0.01),与IFN-γ呈负相关(r=-0.76,P<0.01)。结论 s Tim-3和Galectin-9在布鲁氏菌病患者体内高表达,参与了布鲁氏菌病的发生发展。
To investigate the roles of sTim3 and Galectin-9, as well as related cytokines IFN-γand IL-4 in patients infected with Brucella, the serum levels of sTim-3 and Galectin-9 were detected by ELISA, and the levels of IFN-γand IL-4 were measured by CBA, the correlations of sTim-3 with IFN-γ and IL-4 levels in sera of patients infected with Brucella were analyzed by Pearson correlation test. Compared with healthy controls, sTim-3/ Galeetin-9 and IL-4 expression levels were significantly increased （P〈0.05）, while the level of IFN-γ decreased significantly （P〈0.01） in the brucellosis patients. Pearson analysis showed that there was a positive correlation between sTim-3 and IL-4 （r=0.87, P〈0.01）, while sTim-3 and IFN-γ were negatively correlated （r=-7.60, P〈 0.01）. In conclusion, the increase of sTim-3 and Galectin-9 may be involved in the process of Brueella infection.
作者
庞盼
李明
段亚楠
贾斌
张跃新
甫拉提.热西提
张峰波
丁剑冰
PANG Pan LIMing DUAN Yanan JIA Bin ZHANG Yuexin FULATI · Rexiti ZHANG Fengbo DING Jianbing(Department of Immunology, College of Preclinical Medicine, Xinjiang Medical University, Urumqi 830011, China Central Hospital of Karamay, Karamay 834000, China Department of Infectious Disease Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China Department of Clinical Laboratory, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China)
出处
《免疫学杂志》
CAS
CSCD
北大核心
2017年第1期54-58,共5页
Immunological Journal
基金
国家自然科学基金(81560322)
新疆维吾尔自治区自然科学基金(2015211C100)
新疆医科大学科学创新基金(XYDCX201507)
