摘要
目的探讨盐酸小檗碱、人参皂苷Rb1、黄芩苷及绿原酸对大肠埃希菌和金黄色葡萄球菌的抑菌作用。方法运用96孔板点样法,采用酶标仪测定1 000、500、250、125、62.50、31.25 mg/L浓度下的盐酸小檗碱、人参皂苷Rb1、黄芩苷以及绿原酸对大肠埃希菌以及金黄色葡萄球菌的吸光值,计算抑菌率,比较4种成分对大肠埃希菌和金黄色葡萄球菌的抑菌作用。结果1 000、500、250、125、62.50、31.25 mg/L的盐酸小檗碱、人参皂苷Rb1、黄芩苷以及绿原酸对大肠埃希菌和金黄色葡萄球菌的抑菌效果比较,差异均有统计学意义(P<0.05),且均为1 000 mg/L的抑菌率>500 mg/L的抑菌率>250 mg/L的抑菌率>125 mg/L的抑菌率>62.50 mg/L的抑菌率>31.25 mg/L的抑菌率;同一浓度的不同药物抑菌率比较差异也有统计学意义(P<0.05),对于大肠埃希菌,头孢吡肟的抑菌率最高,其次是人参皂苷Rb1和盐酸小檗碱,最后是绿原酸和黄芩苷。对于金黄色葡萄球菌,头孢吡肟的抑菌率最高,其次是盐酸小檗碱,再次是人参皂苷Rb1,然后是绿原酸,黄芩苷的抑菌效果最差。结论盐酸小檗碱、人参皂苷Rb1、黄芩苷和绿原酸4种中药成分对大肠埃希菌和金黄色葡萄球菌均有一定的抑菌作用,浓度越高抑菌作用越强,同一药物浓度下人参皂苷Rb1和盐酸小檗碱的抑菌效果较好,绿原酸和黄芩苷的抑菌效果较差。
Objective To investigate the bacteriostasis of berberine hydrochloride, ginsenoside Rbl, baicalin and chlorogenic acid on Escherichia coli and Staphylococcus. Methods By 96-pore plate spotting method, microplate reader was used to detect the absorbance of Escheriehia coil and Staphylococcus under the inhibition rate of 1 000,500,250,125, 62.50, 31.25 mg/L of berberine hydrochloride, ginsenoside Rb1, baicalin and chlorogenic acid and calculate the bacteriostasis rate and compared the bacteriostasis of the 4 components on Escherichia eoli and Staphylococcus anreus. Results The antibacterial effect on Escherichia coli and Staphylococcus aureus of 1 000,500,250,125,62. 50,31.25 mg/L of berberine hydrochloride, ginsenosides Rbl, baicalin and chlorogenic acid were statistically significantly different （ P 〈 0.05 ）, inhibition rate of i 000 mg/L 〉 500 mg/L 〉 250 mg/L 〉 125 mg/L 〉 62.50 mg/L 〉 31. 25 mg/L. Under the same concentration, the inhibitory rates of different drugs were also statistically significantly different（ P 〈 0. 05 ）, for Escherichia coli： cefepime had the highest inhibitory rate, followed by ginsenoside Rbl and berberine hydrochloride, the last were chlorogenic acid and baicalin. For Staphylococcus aureus： the inhibitory rate of eefepime was the highest, followed by the hydrochloric acid berberine, the third was ginsenoside Rbl and then the chlorogenic acid, baicalin was the last. Conclusion The 4 kinds of Chinese medicinal components, berberine hydroehloride, ginsenoside Rhl, baicalin and chlorogenic acid, have a certain inhibitory effect, and higher concentration has stronger antibacterial effect. Under the same concentration, the inhibitory effect of ginsenoside Rbl and berberine hydrochloride is better than chlorogenic acid and baicalin.
出处
《医学综述》
2016年第24期4969-4972,共4页
Medical Recapitulate
