摘要
旨在利用SCo T分子标记技术为甜菜指纹图谱构建、遗传多样性分析以及其他分子标记辅助育种提供技术支持。本研究利用单因素实验优化了甜菜SCo T-PCR反应体系。结果表明,在20μL反应体系中,甜菜SCo T-PCR最优反应体系包含2.0μL的10×PCR buffer(含Mg2+)、10 ng的DNA、0.5 U的Taq DNA聚合酶、10μmol/L的引物2μL以及0.1μL的d NTPs(2.5 mmol/L each)。利用优化后的程序对12份糖甜菜品种进行扩增,结果表明,该体系扩增结果稳定、条带清晰,可用于甜菜品种指纹图谱的构建以及其他分子生物学领域的研究。
The objective of the study is to provide technical support for fingerprint construction, genetic diversity analysis and other molecular marker assisted breeding of sugarbeet, with SCo T molecular markers.This sugarbeet SCo T-PCR reaction system was optimized by using the single factor experiment. The resultsshowed that the SCo T-PCR optimal reaction system contained 2.0 μL of 10×PCR buffer（including Mg2 ＋）,10 ng of DNA template, 0.5 U of Taq polymerase, 2 μL of 10 μmol/L SCo T primers and 0.1μL of d NTPs（2.5 mmol/L each） in the 20 μL reaction system. Then, 12 varieties of sugarbeets were amplified using theoptimized reaction system. The results demonstrated that this system was stable and had clear amplified band,so it could be used for constructing fingerprint of sugarbeet and other molecular biology research.
出处
《中国农学通报》
2016年第34期119-122,共4页
Chinese Agricultural Science Bulletin
基金
国家甜菜现代农业产业技术体系建设项目"东北区育种"(CARS-210104)
