摘要
目的:探讨急性非M3髓细胞性白血病(AML non-M3)患者骨髓WT1表达水平的临床意义,了解CD34^+和Auer^+的AML的生物学特征及其对初次诱导缓解率和预后的影响。方法:应用实时荧光定量聚合酶链反应(RQ-PCR)检测92例初诊AML(non-M3)患者骨髓WT1表达水平,分析初次诱导缓解率(CR1)与总生存率(OS)的关系。比较CD34^+和CD34^-以及Auer^+和Auer^-AM L患者在CR1和OS方面的区别。结果:初诊时WT1高表达的AML具有较低的CR1(P<0.05)。CD34^+患者的CR1较CD34^-者低(P<0.05),Auer^+的AML患者CR1较Auer^-AM L患者高(P<0.05)。WT1高表达组的OS低于低表达组,具有显著性差异(P<0.05)。CD34^+组的OS低于CD34^-组,且具有显著差异(P<0.05),Auer^+组与Auer^-组的OS没有明显差异。结论:AML患者骨髓WT1高表达及CD34^+提示患者的预后不良,Auer小体阳性仅与CR1相关,而与OS无关。
Objective：To explore the clinical significance of WT1 expression level in patient with AML non-M3,understand the biological characteristics of Auer^＋ and CD34^＋ AML and its effects on first induced remission rate and prognosis.Methods：The RQ-PCR was used to detect the WT1 expression levels in 92 patients suffering AML non-M3;the relationship between the CR1 and OS was analysed and the differences of CR1 and OS in AML with Auer^＋ and Auer,CD34^＋ and CD34 were compared.Results：AML with WT1 high expression level at first visit had quite lower CR1（P〈0.05）.AML with CD34^＋ had quite lower CR1（P〈0.05）.Compared with Auer^- patients,CR1 of Auer^＋CML patients was higher（P〈0.05）,the OS of AML patients with WT1 high expression level was lower than that of the AML patients with low expression level of WT1,and with significant differences（P〈0.05）,the OS of AML patients with CD34^＋ was lower than that of AML patients with CD34,and with significant differences（P〈0.05）.There was no obvious difference in OS between the AML patients with Auer^＋ and Auer.Conclusion：High expression level of WT1 and CD34^＋ in AML patients suggests the poor prognosis.The Auer positive only relats with CR1,and does not relate with OS.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2016年第6期1670-1674,共5页
Journal of Experimental Hematology
基金
安徽省蚌埠医学院自然科学基金项目(BYKY1440)
安徽省高等学校省级自然科学基金项目(Kj2012A198)
