摘要
目的 分析核酸实时荧光聚合酶链式反应方法检测高危HPV（16和18型分型）（PCR12＋2）联合TCT检查应用于宫颈癌临床筛查中的价值。方法 将进行宫颈癌筛查的480例受检对象行宫颈脱落细胞的薄层液基细胞学（TCT）、核酸实时荧光PCR检测的同时,对HPV16和HPV18进行分型检测（PCR12＋2）、阴道镜活检及组织病理学检查。对检测结果分组（TCT组、PCR12＋2组、TCT联合PCR12＋2）评价、分析。结果 三组患者病理学检查≥宫颈上皮内瘤变Ⅱ级（CIN Ⅱ）比较,差异无统计学意义（χ^2=3.35,P〉0.05）;TCT组阳性率为59.09%,PCR组为64.0%,联合组检测阳性率为94.44%,联合检测的阳性率明显高于单独对HPV16和HPV18进行分型检测的结果,差异具有显著性（P〈0.05）。结论 联合核酸实时荧光PCR方法检测高危HPV（16和18型分型）（PCR12＋2）与TCT联合检查应用于宫颈癌临床筛查的阳性诊断率高,具有更好的准确性。
Objective To analyze the value of nucleic acid real-time polymerase chain reaction method on detection of high risk HPV（ 16 and 18 type） （PCR12＋2） combined with TCT to screen cervical cancer.Methods 480 cases of cervical cancer screening were examined as follows：Thin - layer liquid-based cytology（TCT） of cervical exfoliative cells and real-time PCR detection of nucleic acid, andclassifieation detection（ PCRn＋2） ,colposcopic biopsy and histopathological examination of HPV6 and HPV18.Results There was no significant difference in patho- logical examination of cervical intraepithelial neoplasia （ CIN II） among the three groups （ χ^2= 3.35, P 〉 0.05 ）. The positive rate of each group were as follows ：TCT group 59.09%, PCR group 64.0%, combined group 94.44%, respectively.The positive rate of combined detection was significantly higher than those of HPV16 and HPV18 which were carried out respectively,and the difference was significant（P〈0.05）.Conclusion The real-time PCR assay for detection of high-risk HPV （type 16 and 18） （PCR12＋2）combined with TCT for cervical cancer screening show higherpositive rate and better accuracy for diagnosis.
作者
李海萍
高博文
LI Hal-ping GAO Bo-wen(Department of Gynecological Oncology, Qinghai University Affiliated Hospita Logistics University of PAP)
出处
《青海医学院学报》
CAS
2016年第4期268-272,共5页
Journal of Qinghai Medical College
基金
青海省医药卫生科技项目课题2015年指导性科研课题
