摘要
本研究旨在探索饲粮中泛酸水平对意大利工蜂蜜蜂幼虫发育、机体抗氧化能力及辅酶A（Co A）合成相关酶基因表达的影响,以期得到蜜蜂幼虫阶段饲粮中泛酸的适宜水平。试验选取1日龄意大利蜜蜂工蜂幼虫1 800只,随机分为5组,每组3个重复,每个重复120只。5组工蜂幼虫分别饲喂泛酸实测水平为0.92（对照）、1.22、1.52、1.82、2.12 mg/g的试验饲粮,饲喂至化蛹。分别取5日龄和7日龄幼虫测定体重、体成分、血淋巴生化指标、抗氧化指标、Co A合成相关酶基因的相对表达量,并计算幼虫的化蛹率和羽化率。结果表明：1）饲粮中添加泛酸可显著提高虫体鲜重、干重以及粗脂肪含量（P〈0.05）;当泛酸水平为2.12 mg/g时,幼虫的羽化率显著高于其他组（P〈0.05）。2）饲粮中泛酸水平对5日龄工蜂幼虫血淋巴中总蛋白（TP）、总胆固醇（TCHO）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）含量有显著影响（P〈0.05）,且分别在1.82、1.82、1.22、1.52 mg/g组含量最低。3）5日龄和7日龄虫体总抗氧化能力（T-AOC）以及5日龄虫体超氧化物歧化酶（SOD）活性随饲粮泛酸水平的升高而显著升高（P〈0.05）。4）饲粮泛酸水平显著影响5日龄幼虫泛酸激酶4基因和磷酸泛酰-半胱氨酸脱羧酶基因的相对表达量（P〈0.05）,且均在泛酸水平为1.82 mg/g时相对表达量最高。分别以5日龄虫体的干重和羽化率作拟合曲线,获得意大利蜜蜂工蜂幼虫饲粮中适宜的泛酸水平为1.85~2.01 mg/g。
The objective of this experiment was to research the influences of dietary pantothenic acid level on development,body antioxidant function and the expressions of CoA synthesis related enzyme genes for Apis mellifera ligustica worker bee larvae,and to find the appropriate dietary pantothenic acid level in this stage.A total of 1 800 one-day-old Apis mellifera ligustica worker bee larvae were randomly assigned to 5 groups with 3replicates per group and 120 larvae per replicate.Larvae in 5 groups were fed with experimental diets with0.92,1.22,1.52,1.82 and 2.12 mg/g（measured values） pantothenic acid until pupate,respectively.The body weight,body composition,haemolymph biochemical indices,antioxidant indices and the relative expression levels of CoA synthesis related enzyme genes of 5-and 7-day-old larvae were measured,and then the pupation and eclosion rates were calculated.The results showed as follows：1） pantothenic acid supplementation could significantly increase the fresh weight,dry weight and ether extract（EE） content of larvae（P〈0.05）,and eclosion rate in 2.12 mg/g group was significantly higher than that in other groups（P〈0.05）.2） Dietary pantothenic acid level had significantly influences on total protein（TP）,total cholesterol（TCHO）,high density lipoprotein（HDL） and low density lipoprotein（LDL） contents in haemolymph of 5-day-old larvae（P〈0.05）,meanwhile,they reached the minimum in 1.82,1.82,1.22 and 1.52 mg/g groups,respectively.3）The total anti-oxidant capacity（T-AOC） of 5-and 7-day-old larvae was significantly increased with the dietary pantothenic acid level increasing（P〈0.05）,as well as the superoxide dismutase（SOD） activity of 5-day-old larvae.4） Dietary pantothenic acid level significantly influenced the relative expression levels of pantothenate kinase 4（PANK4） and phosphopantothenoylcysteine decarboxylase（PPCDC） of 5-day-old larvae（P〈0.05）,and both of them reached the maximum in 1.52 mg/g group.Considering dry weight and eclosion rate of 5-day-old larvae to make fitting curves,the appropriate dietary pantothenic acid level for Apis mellifera ligustica worker bee larvae is 1.85 to 2.01 mg/g.
出处
《动物营养学报》
CAS
CSCD
北大核心
2016年第12期3846-3855,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家蜂产业体系建设专项资金(CARS-45)
山东省农业良种工程项目(2014-2016)
关键词
泛酸
意大利蜜蜂
工蜂
幼虫
适宜水平
pantothenic acid
Apis mellifera ligustica
worker bee
larvae
appropriate level
