摘要
目的:研究槲皮素对大肠杆菌和金黄色葡萄球菌生物膜的抑制作用及其对人表皮细胞株HaCaT的增殖影响,探讨槲皮素用于治疗慢性皮肤炎症的潜在可能。方法:常规培养大肠杆菌和金黄色葡萄球菌菌株及HaCaT细胞系,结晶紫染色法检测不同浓度槲皮素处理后24h大肠杆菌及金黄色葡萄球菌生物膜生长水平,公式计算其抑制率;MTT法检测不同浓度槲皮素处理后0,24,48,72h后细胞增殖水平,公式计算其抑制率。结果:槲皮素对大肠杆菌及金黄色葡萄球菌的抑制率均随浓度增大而升高,在400μg/ml和800μg/ml浓度时抑菌效果明显增强,可在24h内达到78%-89%;200,400,800μg/ml浓度的槲皮素对HaCaT细胞生长增殖均无明显抑制作用。结论:适当浓度的槲皮素可明显抑制大肠杆菌及金黄色葡萄球菌生物膜生长,且对HaCaT细胞增殖无明显抑制作用,有望成为光敏性皮肤疾病替代或联合用药的有效药物。
Objective：To study the influence of quercetin on the biofilm of Escherichia Coli and Staphylococcus aureus and the proliferation of human epidermal cell line HaCaT,so as to explore the possibility of quercetin treating chronic dermatitis.Methods：Escherichia Coli,Staphylococcus aureus and HaCaT cells were routinely cultured.Crystal violet assay was used to test the bacterial biofilm proliferation after treatment by different concentration of quercetin for 24 h,and the inhibition rate was calculated by specific formula;MTT assay was used to evaluate the cell proliferation after treatment by different concentration of quercetin for 0,24,48,72 h,and the inhibition rate was calculated.Results：The inhibitory effect of quercetin on both Escherichia Coli and Staphylococcus aureus were obviously increased with the increase of concentration,and the inhibition rate could reach 78%and 89%after 24 htreatment at 400μg/ml and 800μg/ml,respectively.Moreover,the relative HaCaT cell growth rate in 200,400,800μg/ml quercetin treated groups showed no significantly decreases 24hpost-treatment.Conclusion：Quercetin could effectively inhibit the proliferation of Escherichia Coli and Staphylococcus aureus biofilm with no significantly influence on HaCaT cell,which indicated that quercetin could be a potential medicine to treat chronic actinic dermatitis.
作者
韩林
张志
邓晓红
HAN Lin ZHANG Zhi DENG Xiaohong(Dept. of Dermatology Dept. of Neurology, China Resources ＆ Wisco Genenral Hospital ,Wuhan 430080, China)
出处
《武汉大学学报（医学版）》
CAS
2017年第1期11-14,共4页
Medical Journal of Wuhan University
基金
湖北省卫生厅科研基金项目(编号:JX6C-35)
关键词
槲皮素
抗菌
细胞增殖
慢性光化性皮炎
Quercetin
Cell Qroliferation
Biofilm
Chronic Actinic Dermatitis
