摘要
目的探讨大鼠肠道菌群紊乱与卒中后抑郁的关系。方法取清洁级SD大鼠30只,按数字表法分为空白对照组、假手术组、粪菌移植组、假粪菌移植组、卒中后抑郁组5组,每组6只大鼠。对空白对照组大鼠常规饲养,假手术组仅在局部切开皮肤,游离颈动脉后直接缝合切口,粪菌移植组经直肠灌注卒中抑郁患者粪便细菌上清液进行粪菌移植,假粪菌移植组采用等量等渗盐水灌肠,卒中后抑郁组采用单侧颈总动脉不完全结扎法,配合孤养结合利血平注射法造模。在实验进行第7、14、21天通过蔗糖水试验、旷野试验观察其行为学改变,评价动物造模情况。造模成功后,检测血清单胺类神经递质及大鼠肠道细菌群落构成情况。结果 (1)第14、21天,粪菌移植组大鼠糖水消耗量、垂直运动及水平运动明显少于空白对照组和假粪菌移植组(均P<0.05),卒中后抑郁组大鼠糖水消耗量明显少于空白对照组和假手术组(均P<0.05)。(2)粪菌移植组大鼠大脑中肾上腺素、多巴胺、5-羟色胺含量较空白对照组和假粪菌移植组明显降低(均P<0.05);卒中后抑郁组大鼠大脑中肾上腺素含量较空白对照组明显降低(P<0.05),多巴胺、5-羟色胺含量较空白对照组和假手术组明显降低(均P<0.05)。(3)粪菌移植组大鼠肠道中普雷沃菌属、颤克杆菌属含量较空白对照组和假粪菌移植组明显降低(均P<0.05),大肠杆菌/志贺杆菌属含量较假粪菌移植组明显降低(P<0.05),卒中后抑郁组大鼠肠道中普雷沃菌属、大肠杆菌/志贺杆菌属含量较空白对照组和假手术组明显减少,颤克杆菌属含量较空白对照组明显减少(P<0.05)。结论大鼠脑内单胺类神经递质水平变化及肠道菌群构成的改变,尤其是大肠杆菌/志贺杆菌属、普雷沃菌属、颤克杆菌属比例的减低,在一定程度上参与了卒中后抑郁大鼠模型的病情变化。
Objective To investigate the relationship between intestinal flora disturbance and depression after stroke in rats. Methods Thirty clean grade SD rats were selected. They were randomly divided into 5 groups： blank control,sham operation,fecal bacteria transplantation,false fecal bacteria transplantation,and poststroke depression（ n = 6 in each）. The rats in the blank control group were fed conventionally; those in the sham operation group were locally incised the skin and directly sutured after the free carotid artery; those in the fecal bacteria transplantation group conducted fecal bacteria transplantation via rectal infusion of fecal bacteria supernatant in stroke patients with depression,those in the false fecal bacteria transplantation group were treated with the same amount of isotonic saline enema; and those in the poststroke depression group were modeled with unilateral carotid artery incomplete ligation and solitary raise combined with reserpine injection. The behavioral change was observed through the sucrose water test and field test at day 7,14,and 21. The animal modeling was evaluated. After the successful modeling,the changesof the serum monoamine neurotransmitters and the structure of intestinal bacterial community in rats were detected. Results（ 1） At day 14 and 21,the sucrose consumption,horizontal movement,and vertical movement of the rats in the fecal bacteria transplantation group were significantly less than those of the blank control group and the false fecal bacteria transplantation group（ all P 〈0. 05）.（ 2） The levels of adrenaline,dopamine,and 5-serotonin in the rat brain of the fecal bacteria transplantation group were significant lower than those of the blank control group and the false fecal bacteria transplantation group（ all P 〈0. 05）. The level of adrenaline in rat brain of the poststroke depression group was significantly lower than that in the blank control group（ P 〈0. 05）. The levels of dopamine and 5-serotonin were significantly lower than those of the sham operation group（ all P 〈0. 05）.（ 3） The levels of rat intestinal Prevotella spp. and AF g Bacilli in fecal bacteria transplantation group were significantly lower than those in the blank control group and false fecal bacteria transplantation group（ all P 〈0. 05）. The levels of Escherichia coli / Shigellas were significantly lower than those of the false fecal bacteria transplantation group（ P 〈0. 05）. The levels of rat intestinal Prevotella spp.,Escherichia coli,and Shigella in the poststroke depression group were significantly lower than those in the blank control group and the sham operation group. The level of Bacillus spp. was significantly less than the blank control group（ P 〈0. 05）. Conclusion The changes of the level of monoamine neurotransmitters in brain and the changes in the structure of intestinal flora,especially the decrease of the proportions of Escherichia coli / Shigella,Prevotella spp,and Bacillus spp,participate in the change of the condition of poststroke depression rat model to a certain degree.
出处
《中国脑血管病杂志》
CAS
CSCD
北大核心
2016年第12期644-649,共6页
Chinese Journal of Cerebrovascular Diseases
基金
国家自然科学基金项目(81470188
81373840
81673832)
陕西省教育厅项目(14JK1197)
陕西省中医药管理局项目(13-LC073)
陕西省科技厅社发公关项目(2016SF-375)
关键词
卒中
抑郁症
肠道菌群
紊乱
Stroke
Depression
Intestinal flora
Disturbance
