摘要
采用气相色谱-质谱(GC-MS)技术对茅台酱香型酒糟与酒曲石油醚萃取部分的脂溶性成分进行分析,通过K-B纸片法评价茅台酱香型酒糟与酒曲脂溶性成分的体外抑菌活性。结果表明,从茅台酱香型酒糟与酒曲的脂溶性成分中分别鉴定出18种化合物,占总峰面积的100%。其中烷烃类(50.19%)、酯类(49.58%)为茅台镇酒糟主要脂溶性成分类型,茅台镇酒曲脂溶性成分主要为烷烃类化合物(95.79%)。抑菌实验表明,茅台酱香型酒曲石油醚提取物对金黄色葡萄球菌(SA)、耐甲氧西林金黄色葡萄球菌(MRSA)和产超广谱β-内酰胺酶的金黄色葡萄球菌(ESBLs-SA)均有抑制作用,其中对MRSA抑制作用最好,抑菌圈直径为8 mm。
Liposoluble components from Moutai distiller's grains and Jiuqu with petroleum ether extraction of were analyzed by GC-MS. The in vitro antibacterial activity was determined by K-B method. The results showed that 18 compounds were identified respectively from Moutai distiller's grains and Jiuqu, accounting for 100% of the total detected constituents. Alkanes (50.19%) and esters (49.58%) were the main compound types in Moutai distiller's grains; and alkanes (95.79%) were the main compound type in Jiuqu. The extract from Jiuqu with petroleum ether extraction had a significant antibacterial effect on Staphylococcus aureus, methcillin-resistant S. aureus (MRSA) and extended spectrum β-lactamase S. aureus (ESBLs-SA), the effect on MRSA was the most significant, and the inhibition zone was 8 mm.
作者
王兴东
牟明月
康文艺
张前军
周清娣
卫钢
WANG Xingdong MU Mingyue KANG Wenyi ZHANG Qianjun ZHOU Qingdi WEI Gang(College of Chemistry and Engineering, Guizhou University, Guiyang 550025, China Kweichow Moutai Co., Ltd., Renhuai 564500, China Pharmaceutical College o fHenan University, Kaifeng 475004, China College of Chemistry, The University of Sydney, New South Wales 2006, Australia CSlRO Manufacturing, 218, New South Wales 2070, Australia)
出处
《中国酿造》
CAS
北大核心
2016年第12期44-47,共4页
China Brewing
基金
教育部春晖计划项目(Z2015003)