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基质辅助激光解吸附电离飞行时间质谱技术对痤疮患者痤疮丙酸杆菌的鉴定 被引量：11

Identification Detection of Propionibacterium Acnes Isolated from Patients with Acne by Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
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摘要 目的了解基质辅助激光解吸附电离飞行时间质谱(MALDI-TOF MS)技术鉴定痤疮丙酸杆菌的效果。方法应用MALDI-TOF MS技术对痤疮患者皮损分离的91株菌株进行鉴定。结果 70株为痤疮丙酸杆菌,19株为其他厌氧菌或兼性厌氧菌(表皮葡萄球菌8株、颗粒丙酸杆菌3株、头状葡萄球菌2株、蜡样芽胞杆菌1株、产芽孢梭状芽孢杆菌1株、贪婪丙酸杆菌1株、克氏柠檬酸杆菌1株、毗邻颗粒链菌1株、结核硬脂酸棒状杆菌1株),2株无鉴定结果。结论 MALDI-TOF MS技术鉴定痤疮丙酸杆菌快速而准确,值得选用。 Objective To understand the clinical performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry （ MALDI-TOF MS） system in the identification of Propionibacterium ac- Mes. Methods Ninty-one clinical strains were isolated and cultured, and identified by MALDI-TOF MS. Results Identifiy the 9l strains by MALD1-TOF-MS, 70 strains were recovered as Propionibacterium acMes, 19 trains were other anaerobic or facuhative anaerobes, and 2 have no reliable identifica- tion. Conclusion The MALDI-TOF MS system maybe used to identify propionibacterium acMes rapidly and accurately, thus deserving popularization.
作者 凌勇活 孙菲 李明 党文强 阮建波 LING Yong-huo SUN Fei LIMing DANG Wen-qiang RUAN Jian-bo(Dongguan Hospital Affiliated to Medical College of Jinan University, Dongguan523905, China Guangzhou KingMed Diagnostics Center Co. Ltd. , Guangzhou 510000, China)
机构地区 暨南大学医学院附属东莞医院 广州金域医学检验中心有限公司
出处 《中国皮肤性病学杂志》 CAS CSCD 北大核心 2017年第1期98-99,103,共3页 The Chinese Journal of Dermatovenereology
基金 东莞市社会科技发展项目(2013108101042)
关键词 基质辅助激光解吸附电离飞行时间质谱 痤疮丙酸杆菌 Matrix-assisted laser desorption ionization time-of-flight mass spectrometry Propionibacterium acMes
分类号 R758.73 [医药卫生—皮肤病学与性病学]
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中国皮肤性病学杂志
2017年 第1期

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