摘要
目的在细胞水平研究松弛素的抗肝纤维化作用,并初步探讨其分子机制,为肝纤维化的治疗提供实验依据。方法体外培养大鼠肝星状细胞(HSC-T6),分别用20、50、100ng/mL重组人松弛素-2(recombinant human relaxin-2,RLX-2)干预,采用噻唑蓝(MTT)比色实验检测RLX-2对HSC-T6增殖的影响,双抗体夹心酶联免疫吸附法(ELISA)检测对照组及RLX-2作用48h后的细胞培养基上清液Ⅰ型胶原水平,利用逆转录酶-聚合酶链反应(RT-PCR)技术检测药物干预48h的结缔组织生长因子(CTGF)、转化生长因子-β1(TGF-β1)mRNA表达情况。结果 RLX-2对HSC的增殖具有抑制作用,可降低HSCⅠ型胶原水平,RLX-2抑制HSC的CTGF mRNA表达,对HSC的TGF-β1mRNA表达无明显影响。结论 RLX-2可能通过抑制体外培养大鼠HSC细胞的增殖、Ⅰ型胶原及CTGF mRNA的表达而发挥抗大鼠肝纤维化作用。
Objective To validate that relaxin can resist hepatic fibrosis at the cellular level and explore its molecular mechanism in order to provide experimental basis for the treatment of liver cirrhosis. Methods Cultured HSC-T6s were treated with different concentrations （20, 50 and 100 ng/mL） of recombinant human relaxin-2 （RLX-2）. The proliferation of HSC-T6 was measured by MTT colorimetric assay. The content of type Ⅰ collagen in the cell culture supernatant of each group was detected by ELISA at 48 h of drug intervention; RT-PCR was used to detect the mRNA expressions of CTGF and TGF-β1 in HSC-T6 at 48 h of drug intervention. Results RLX-2 inhibited the proliferation of HSC and reduced type Ⅰ collagen content of HSC cells. It also inhibited the CTGF mRNA expression of HSC, but did not have a significant effect on the expression of TGF-β1 mRNA. Conclusion In the experiment of culturing HSC-T6 in vitro, RLX-2 may play a role in rat liver fibrosis by inhibiting cell proliferation and type Ⅰ collagen and CTGF mRNA expressions.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2017年第1期44-47,57,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
