摘要
目的 :以聚己内酯(polycaprolactone,PCL)为静电纺丝材料,制备载有辛伐他汀(simvastatin,SMV)的纤维膜,分析其药物缓释特征,构建具有SMV缓释功能的支架材料,用于牙周组织工程。方法:将SMV和PCL溶解于三氯甲烷,得到均相溶液,制备载SMV的PCL纤维膜作为实验组,以纯PCL纤维膜作为对照组。对所得纤维膜采用扫描电子显微镜和广角X射线衍射进行形貌和表征分析,通过紫外分光光度计测定纤维膜释放至介质中的SMV,绘制释放曲线,分析载SMV纤维膜的药物缓释特征。采用SPSS 17.0软件包对数据进行统计学分析。结果:SMV/PCL纤维膜由纳米级静电纺丝构成,SMV与PCL结合较好,药物包裹于纤维之中;累计SMV释放量与时间接近线性相关,能够实现药物的长效缓释。结论:通过静电纺丝技术构建SMV/PCL纳米纤维膜,具有良好的药物缓释作用,有望作为支架材料应用于牙周组织工程。
PURPOSE： To prepare electrospun simvastatin/polycaprolactone （SMV/PCL） membrane scaffolds and to evaluate the release properties of this formulation. METHODS： Electrospun SMV/PCL membrane scaffolds were prepared as the experimental group, and electrospun PCL membrane as the control group. The morphology and characteristics of membrane surface were determined by scanning electron microscopic （SEM） and X-ray diffraction （XRD）. The release profile of SMV was determined using an ultraviolet-visible spectrometer. The data were analyzed statistically with SPSS 17.0 software package. RESULTS： SEM and XRD indicated that SMV/PCL nanofibers were successfully electrospun and SMV was encapsulated into the fibers. In vitro drug release studies showed that simultaneous SMV release, being nearly linear with time, was achieved and sustained SMV release was prolonged to 28 days. CONCLUSIONS： Electrospun SMV/ PCL nanofiber membranes demonstrate sustained dnag release properties, suggesting their potential applicability as prospective scaffolds in periodontal regeneration.
出处
《上海口腔医学》
CAS
CSCD
2016年第6期673-677,共5页
Shanghai Journal of Stomatology
基金
上海市自然科学基金(15ZR1435900)
关键词
静电纺丝
辛伐他汀
缓释
牙周再生
Electrospinning
Simvastatin
Controlled release
Periodontal regeneration
