摘要
利用农杆菌介导法,以东北地区种植的9个大豆基因型和国外品种Williams82的子叶节为外植体,将抗虫基因cry1Iem转入栽培大豆品种中。共转化外植体1 459个,获得84棵再生植株。采用除草剂叶片涂抹法、PCR及Bar蛋白试纸条检测法对得到大豆再生植株进行鉴定,转cry1Iem基因大豆T_0阳性植株为61株。对部分T_1转基因植株的遗传分析表明,外源基因能够稳定遗传到下一代。通过对部分T_1阳性转基因植株进行Southern blot分析,证明目的基因片段均已整合到受体大豆的基因组DNA中,单拷贝率为22%左右。对获得的部分T_2材料进行了室内抗大豆食心虫鉴定,有2份转基因材料抗性较对照显著提高。
Insect-resistant gene cry1Iem was transformed into nine soybean genotypes of northeastern China and Williams 82 by Agrobacterium-mediated of cotyledonary nodes. 84 putative transgenic soybean plants were obtained and 61 positive transgenic soybean plants were identified by coating with leaves herbicide, bar protein quick dip stick and PCR analysis. Southern blot analysis revealed that cry1Iem gene had been integrated into the genomes of T1 transgenic soybean lines. The T1 lines with single-copy cry1Iem gene accounted for 22% of the total. In addition, the resistance of two transgenic soybean lines to soybean pod borer was significantly higher than that of the control.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2016年第6期757-763,共7页
Chinese Journal of Oil Crop Sciences
基金
国家转基因生物新品种培育重大专项(2016ZX08004-004)
