摘要
背景:胰岛素样生长因子1作为生长激素中的主要活性分子,发挥着多种生物学功能,其中便包括对认知功能改善和抗细胞凋亡作用。目的:检测Lewis侏儒大鼠颞叶皮质中生长激素、胰岛素样生长因子1的表达,观察不同浓度生长激素对海马神经干细胞分化的影响。方法:将11月龄(成年)、20月龄(老年)Lewis侏儒及正常野生型大鼠断头处死,迅速开颅,在预冷过的生理盐水中分离出颞叶皮质,Western blot检测生长激素、胰岛素样生长因子1表达。在分离、纯化和鉴定了大鼠海马神经干细胞后,观察用不同浓度(10,30,90μg/L)的生长激素培养96 h后对海马神经干细胞分化的影响。结果与结论:1成年、老年Lewis侏儒大鼠颞叶皮质中生长激素的表达量与正常同龄野生型大鼠相比,差异均无显著性意义(P>0.05);成年、老年Lewis侏儒大鼠颞叶皮质中胰岛素样生长因子1较野生型大鼠明显升高(P<0.05);成年雌性侏儒大鼠生长激素的表达量显著低于成年雄性侏儒大鼠(P<0.05);2荧光免疫组化结果显示分散的SD大鼠海马神经干细胞和前体细胞,在加入30μg/L生长激素培养96 h后,βⅢ-tubulin阳性的神经元比例较对照组显著升高(P<0.05),但10μg/L和90μg/L生长激素处理组βⅢ-tubulin阳性神经元的比例与对照组差异无显著性意义;3结果表明,Lewis侏儒大鼠和野生大鼠颞叶皮质中均有生长激素、胰岛素样生长因子1的表达,这种表达独立于垂体生长激素和循环性胰岛素样生长因子1;生长激素具有促进海马神经干细胞和前体细胞向神经元分化的功能。
BACKGROUND：Insulin-like growth factor-1（IGF-1）, a main active factor in growth hormone （GH）, plays various biological functions, such as improving cognitive ability and anti-apoptotic action. OBJECTIVE：To detect the expressions of GH and IGF-1 in the temporal cortex of Lewis dwarf rats, and to explore the effect of different concentrations of GH on the differentiation of hippocampal nerve stem cels （NSCs）. METHODS：Lewis dwarf rats aged 11（adult） and 20 （senile） month olds and normal wild-type rats were euthanized by decapitation, underwent the craniotomy quickly, and the temporal cortex in the cold saline was extracted. GH and IGF-1 levels were detected using western blotting. After isolation, purification and identification of the rat hippocampal NSCs, the effect of GH in different concentrations （10, 30, 90μg/L） on the NSCs differentiation was determined at 96 hours after culture. RESULTS AND CONCLUSION：The GH level in the temporal cortex did not differ significantly among rats （P 〉 0.05）. While the IGF-I level in the temporal cortex of Lewis dwarf rats was significantly higher than that of the wild-type rats （P 〈 0.05）. The GH level in the temporal cortex of adult female Lewis dwarf rats was significantly lower than that of the male rats （P 〈 0.05）. Immunofluorescence showed that the proportion ofβ III-tubulin-positive neurons was significantly higher than that in the control group （P 〈 0.05） after the hippocampal NSCs and precursor cels cultured for 96 hours with GH （30μg/L）, but there was no significant difference between the control group and treatment group with GH of 10 or 90μg/L. These results suggest that GH and IGF-I are expressed in the temporal cortex of both Lewis dwarf and wild-type rats which are independent from pituitary GH and the peripheral circulating IGF-1. Additionaly, GH can promote the differentiation of hippocampal NSCs and precursor cels into neurons.
出处
《中国组织工程研究》
CAS
北大核心
2016年第51期7690-7696,共7页
Chinese Journal of Tissue Engineering Research
