摘要
为给仁用杏分子育种提供理论依据,培育仁用杏晚花新品种,通过Illumina Hi SeqTM2000高通量测序——转录组测序(RNA-seq)技术,对采于花芽萌动前后3个发育时期的仁用杏花芽进行转录组测序。经组装分析获得43 469条unigenes;将所得到的unigenes与基因库数据进行比较,对unigenes进行功能注释,共22 874条unigenes获得功能注释,并预测出21 910条unigenes蛋白编码区;将3个发育时期仁用杏花芽的花转录组通过GO功能分类,将17 237条unigenes分别归类为46个GO Term;通过将所有unigenes与KOG数据库比对,共有8 379条unigenes与其它植物相似,归为26类,未知功能的unigenes有409条,预测这些基因为仁用杏的新基因;通过与KEGG数据库比对,共获得6 203条unigenes,参与259个代谢途径,其中与植物节律代谢通路相关的unigenes有33条。
In order to provide a theoretical basis for molecular breeding of kernel apricot and to cultivate new varieties with the late florescence characteristic, with the platform of Illumina HiSeq XM 2000 high-throughput sequencing - the transcriptome sequencing （RNA - seq） technology, transcriptomes in blossom bud of kernel apricot were sequenced, which were picked at three development stages of blossom buds. A total of 43 469 unigenes were generated through assembly annotation. All unigenes were compared with the gene database, function annotations of 22 874 unigenes were got, and the coding sequences of 21 910 unigenes were predicted. The transcriptomes in kernel apricot blossom buds at three development stages were classified based on GO function, and 17 237 unigenes were classified into 46 GO terms. All uuigenes were compared with the KOG database, there were 8 379 unigenes similar to other plants which were classified to 26 terms, and 409 unigenes were unkown function, which maybe new genes in kernel apricot. By compared with KEGG database, 6 023 unigenes were collected, which involved into 259 metabolism pathways, and there were 33 unigenes involving in to circadian rhythm metabolism pathways.
作者
宋猜
姜仲茂
李慧
乌云塔娜
SONG Cai JIANG Zhong-mao LI Hui Wuyuntana(Non-Timber Research and Development Center, CAF, Zhengzhou 450003, Henan, Chin)
出处
《经济林研究》
北大核心
2016年第4期14-19,共6页
Non-wood Forest Research
基金
“十二五”科技支撑项目“仁用杏和巴旦杏高效生产关键技术研究与示范”(2013BAD14B02)
关键词
仁用杏
花期
转录组分析
数字基因表达谱
开花途径
kernel apricot
florescence
transcriptome analysis
digital gene expression profiling
blossoming pathway
