摘要
目的:研究苗药理气活血滴丸对心肌缺血再灌注损伤(MIRI)模型大鼠的保护作用。方法:将60只大鼠随机分为假手术组、模型组、辛伐他汀组(阳性对照,40 mg/kg)和理气活血滴丸低、中、高剂量组(43.75、87.50、175.00 mg/kg),每组10只。各给药组大鼠每天ig相应药物1次,连续10 d;假手术组和模型组大鼠ig等体积生理盐水。末次给药45 min后,除假手术组外其余各组大鼠均复制MIRI模型。复灌后监测大鼠心律失常情况;采用苏木精-伊红染色观察心肌组织病理学变化;采用酶动力学法测定血清中心肌酶[肌酸激酶(CK)、乳酸脱氢酶(LDH)]活性;采用酶联免疫吸附法测定血清中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)含量。结果:与假手术组比较,模型组大鼠心律失常程度较高,心肌组织损伤严重,血清中CK、LDH活性及TNF-α、IL-6含量均明显升高(P<0.01)。与模型组比较,辛伐他汀组和理气活血滴丸中、高剂量组大鼠心律失常程度降低,心肌组织病理损伤得到改善,血清中CK、LDH活性及TNF-α、IL-6含量均显著降低(P<0.01),且理气活血滴丸高剂量组与辛伐他汀组各指标水平接近(P>0.05)。结论:理气活血滴丸对MIRI模型大鼠具有一定保护作用,其机制可能与减少心肌细胞心肌酶的漏出、降低血清中TNF-α和IL-6含量、抑制炎性损伤有关。
OBJECTIVE：To investigate the protective effect of Miao medicine Liqi huoxue dropping pills on myocardial ischemia-reperfusion injury（MIRI）model rats. METHODS：60 rats were randomized into sham operation group,model group,simvastatin group（positive control,40 mg/kg）and Liqi huoxue dropping pills low-dose,medium-dose and high-dose groups（43.75,87.50,175.00 mg/kg）,with 10 rats in each group. Treatment groups were given relevant medicine intragastrically once a day for10 days. Sham operation group and model group were given constant volume of normal saline intragastrically. 45 min after last administration,MIRI model was induced in those groups except for sham operation group. The situation of cardiac arrhythmia in rats was monitored after reperfusion. The myocardium tissue morphology was observed by hematoxylin-eosin（HE）staining;the activity of myocardial [creatine kinase（CK）and lactate dehydrogenase（LDH）] in serum were measured by enzyme dynamics method;the contents of tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6）in serum were detected by enzyme linked immunosorbent assay（ELISA）. RESULTS：Compared with sham operation group,rats in model group suffered from more serious arrhythmia and myocardial tissue damaged,and the activities of CK and LDH and the contents of TNF- α and IL- 6 were increased significantly（P〈0.01）. Compared with model group,arrhythmia degrees of rats were decreased in simvastatin group and Liqi huoxue dropping pills medium-dose and high-dose groups,and pathological changes of myocardial tissue was improved,while the activities of CK and LDH and the contents of TNF-α and IL-6 in serum were decreased significantly（P〈0.01）;those indexes of Liqi huoxue dropping pills high-dose group were close to those of simvastatin group（P〉0.05）. CONCLUSIONS：Liqi huoxue dropping pills havethe protective effect on MIRI model rats,and its mechanism may be related to decreasing myocardial enzyme leaking of cardiac muscle cell,decreasing the serum contents of TNF-α and IL-6 and inhibiting the inflammatory injury.
出处
《中国药房》
CAS
北大核心
2017年第1期76-79,共4页
China Pharmacy
基金
贵阳中医学院博士科研项目启动基金(No.中医研〔2015〕017)
贵阳中医学院中西医结合基础学科资助项目(No.黔学位合字ZDXK〔2016〕25号)
关键词
苗药
理气活血滴丸
心肌缺血再灌注损伤
心肌酶
肿瘤坏死因子α
白细胞介素6
大鼠
Miao medicine
Liqi huoxue dropping pills
Myocardial ischemia-reperfusion injury
Myocardial enzyme
Tumor necrosis factor-α
Interleukin-6
Rats
