摘要
为探索娄彻氏链霉菌(D74)和密旋链霉菌(Act12)及其混合菌剂对玉米生长的影响,采用皿内发芽试验、沙培试验及小区试验观察供试链霉菌无细胞发酵滤液及活菌制剂种子包衣对玉米生物学性状、叶片诱导酶活性、光合作用、穗性状、产量及品质的影响.结果表明:供试链霉菌无细胞发酵滤液处理玉米种子可促进玉米胚根、胚轴及幼苗生长,提高玉米幼苗叶片诱导酶活性.D74发酵液稀释1000倍处理可使玉米胚根长度、胚轴长度及须根数较对照分别增加43.4%、26.4%和100.7%(P<0.05);D74原液可使玉米叶片多酚氧化酶(PPO)活性较对照增加40.2%.Act12发酵液稀释100倍处理可使玉米胚根长度、胚轴长度和须根数较对照分别增加36.3%、36.3%和117.5%(P<0.05),玉米幼苗总鲜质量、根系鲜质量分别较对照增加31.1%、36.6%(P<0.05);Act12稀释10及1000倍处理玉米种子可使玉米叶片PPO活性分别较对照增加38.1%和39.5%(P<0.05).混合菌剂种子包衣具有以下作用:1)促进玉米根系发育;2)显著增强叶片光合能力;3)显著改善穗性状并提高籽粒产量;4)显著促进籽粒灌浆速度;5)明显提高灌浆期玉米叶片诱导酶活性.表明供试链霉菌制剂包衣玉米种子可显著影响玉米的生物学特性、光合生理及生化代谢,刺激根系发育,促进玉米生长,提高产量.
This paper was mainly to explore the effects of two Streptomyces strains （S. roche D74 and S. pactum Actl2） and their mixed actinomycetes agent on maize growth. Petri dish germination test, sand culture trial and plot trial were performed to determine the biological properties, leaf inducible enzyme activities, photosynthesis, ear characteristics, grain yield and quality of maize after seed soaking with acellular culture filtrate of D74 or Act 12 and seed coating with the mixed actinomycetes agent of two Streptomyces stains. The result showed that the seed soaking treatment signifi- cantly contributed to hypocotyls, radical and seedling growth, and increased the leaf inducible enzyme activities of maize seedlings. The 1000-fold dilution of D74 improved hypocotyl length, radicle length, and fibrous root number by 43.4%, 26.4%, and 100.7% （P〈0.05） , respectively, whereas the undiluted solution of D74 improved leaf polyphenol oxidase （PPO） activity by 40.2% （P 〈0.05）. The 1000-fold dilution of Actl2 improved hypocotyl length, radicle length, and fibrous root number by 36.3%, 36.3%, and 117.5% （P〈0.05）, the total fresh mass and root fresh mass by 31.1% and 36.6%, respectively （P〈0.05）. The 10-fold and 1000-fold dilutions of Actl2 im- proved leaf PPO activity by 38.1% and 39.5%, respectively （P〈0.05）. The seed coating treatment showed the following significant effects, compared with the control： 1 ） Improving the root development. 2） Enhancing the leaf photosynthesis. 3 ） Improving the ear characteristics and grain yield. 4） Promoteing the grain filling. 5） Improving the leaf inducible enzyme activities. The results indi-cated that seed coating with the mixed actinomycetes agent of two Streptomyces strains could significantly affect the biological characteristics, photosynthesis and biochemical metabolism of maize seedlings, stimulate root development, promote plant growth, and improve grain yield of maize.
出处
《应用生态学报》
CAS
CSCD
北大核心
2017年第1期315-326,共12页
Chinese Journal of Applied Ecology
基金
国家科技支撑计划项目(2012BAD14B11)资助~~
关键词
放线菌剂
生物量
光合作用
种子包衣
根系发育
actinomycetes agent
biomass
photosynthesis
seed coating
root growth.
