摘要
目的回顾性分析8例抗-P1标本,为抗-P1引起的输血相容性检测困难提供解决方案。方法血型鉴定采用强生Auto VUE玻璃珠卡式法,正反定型不一致样本用试管法进行复查;抗体筛查用强生Auto VUE抗人球卡进行检测,阳性样本用Diana抗人球卡做抗体鉴定;试管法鉴定被检者红细胞上是否存在P1抗原,必要时检测反定试剂红细胞是否存在P1抗原;统计8例标本配血相合率。结果 8例抗筛阳性标本均导致ABO正反定型不符,抗体鉴定结果均为抗-P1,配血相合率为14%-67%。结论抗-P1引起正反定型不一致时,应使用P1抗原阴性试剂红细胞进行血型反定型,以确保结果的准确性。
Objective To retrospectively analyze eight cases of anti-P1 samples,and to provide a solution for the difficulties in detection of blood transfusion compatibility caused by anti-P1. Methods ABO blood group was first determined by Johnson Vision Auto VUE glass bead card. The inconsistent samples of forward and reverse blood group were analyzed by tube method. Antibody screening was performed with the Auto VUE anti-human card. Positive samples were identified by Diana anti-human card. If the presence of P1 antigen on the red blood cells were detected,if necessary,the presence of P1 antigen was inspected with reagent red blood cells. Statistics was applied to determine the cross-matching rates in eight cases. Results All eight samples were screened positive for antibodies,which led to forward and reverse blood typing inconsistency. Antibody identification results were all anti-P1.The cross-matching consistent rates were 14%-67%. Conclusion To accurately determine whether the blood group incompatibility is caused by anti-P1,reverse blood grouping with P1 antigen negative reagent red blood cells should be employed.
出处
《中国输血杂志》
CAS
北大核心
2016年第11期1241-1243,共3页
Chinese Journal of Blood Transfusion
关键词
血型鉴定
不规则抗体鉴定
抗-P1
blood grouping
irregular antibody identification
anti-P1
